Honma Kazue, Mochizuki Kazuki, Goda Toshinao
Laboratory of Nutritional Physiology, The University of Shizuoka, Graduate School of Nutritional and Environmental Sciences and COE 21, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan.
Biochem Biophys Res Commun. 2007 Jun 15;357(4):1124-9. doi: 10.1016/j.bbrc.2007.04.070. Epub 2007 Apr 19.
A diet with a high carbohydrate/fat ratio enhances jejunal SI gene expression. Using ChIP assay, we revealed that the acetylation of histone H3 on transcriptional region and H4 on promoter region, respectively, of mouse SI gene are high. The acetylation of histone H3 and H4 as well as binding of HNF-1 and Cdx-2 on SI gene, was enhanced by increase in carbohydrate/fat ratio in the diet. These suggest that induction of SI gene by the diet rich in carbohydrate is associated with acetylation of histone H3 and H4 as well as binding of HNF-1 and Cdx-2 on SI gene.
高碳水化合物/脂肪比例的饮食可增强空肠蔗糖酶异麦芽糖酶（SI）基因的表达。通过染色质免疫沉淀（ChIP）试验，我们发现小鼠SI基因转录区域的组蛋白H3和启动子区域的组蛋白H4的乙酰化水平分别较高。饮食中碳水化合物/脂肪比例的增加可增强组蛋白H3和H4的乙酰化以及肝细胞核因子-1（HNF-1）和尾型同源盒转录因子2（Cdx-2）与SI基因的结合。这些结果表明，富含碳水化合物的饮食对SI基因的诱导作用与组蛋白H3和H4的乙酰化以及HNF-1和Cdx-2与SI基因的结合有关。
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