Bouchard C, Leon A S, Rao D C, Skinner J S, Wilmore J H, Gagnon J
Physical Activity Sciences Laboratory, Laval University, Québec, Canada.
Med Sci Sports Exerc. 1995 May;27(5):721-9.
The HERITAGE family study (HEalth, RIsk factors, exercise Training And GEnetics) will document the role of the genotype in the cardiovascular, metabolic, and hormonal responses to aerobic exercise training. A consortium of five universities in the United States and Canada are involved in carrying out the study. A total of 90 Caucasian families and 40 African-American families with both parents and three or more biological adult offspring are being recruited, tested, exercise-trained in the laboratory with the same program for 20 wk, and re-tested. Oxygen uptake, respiratory exchange ratio, blood pressure, heart rate, cardiac output, blood lactate, glucose, and free-fatty acids are measured during exercise, and maximal oxygen uptake is determined before and after training. Plasma lipids, lipoproteins and apoproteins, glucose and insulin response to an intravenous glucose load, plasma sex steroids and glucocorticoids, and body fat and fat distribution are assessed. Dietary and activity habits and other life style components are assessed by questionnaires, prior to, during, and after training. A variety of genetic analyses will be undertaken, including heritability studies and major gene effects, for each phenotype and its response to regular exercise. Cell lines are established, and DNA sequence variation at a variety of molecular markers will be determined for association and linkage studies.
“遗传因素对运动训练反应的影响”（HERITAGE）家族研究（健康、风险因素、运动训练与遗传学）将记录基因型在有氧运动训练的心血管、代谢和激素反应中的作用。美国和加拿大的五所大学组成的联盟参与了这项研究。目前正在招募、测试总共90个白人家庭和40个非裔美国人家庭，这些家庭均有父母以及三个或更多亲生成年子女，对他们进行实验室测试，按照相同方案进行20周的运动训练，之后再次进行测试。在运动过程中测量摄氧量、呼吸交换率、血压、心率、心输出量、血乳酸、血糖和游离脂肪酸，并在训练前后测定最大摄氧量。评估血浆脂质、脂蛋白和载脂蛋白、静脉注射葡萄糖负荷后的血糖和胰岛素反应、血浆性类固醇和糖皮质激素，以及身体脂肪和脂肪分布。在训练前、训练期间和训练后，通过问卷调查评估饮食和活动习惯以及其他生活方式因素。将针对每种表型及其对规律运动的反应进行各种遗传分析，包括遗传力研究和主要基因效应分析。建立细胞系，并确定多种分子标记处的DNA序列变异，以进行关联和连锁研究。
