Division of Cardiovascular Medicine.
CardioVascular Institute, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.
JCI Insight. 2023 Apr 10;8(7):e165867. doi: 10.1172/jci.insight.165867.
Regular exercise leads to widespread salutary effects, and there is increasing recognition that exercise-stimulated circulating proteins can impart health benefits. Despite this, limited data exist regarding the plasma proteomic changes that occur in response to regular exercise. Here, we perform large-scale plasma proteomic profiling in 654 healthy human study participants before and after a supervised, 20-week endurance exercise training intervention. We identify hundreds of circulating proteins that are modulated, many of which are known to be secreted. We highlight proteins involved in angiogenesis, iron homeostasis, and the extracellular matrix, many of which are novel, including training-induced increases in fibroblast activation protein (FAP), a membrane-bound and circulating protein relevant in body-composition homeostasis. We relate protein changes to training-induced maximal oxygen uptake adaptations and validate our top findings in an external exercise cohort. Furthermore, we show that FAP is positively associated with survival in 3 separate, population-based cohorts.
定期锻炼会带来广泛的有益影响，越来越多的人认识到，运动刺激的循环蛋白可以带来健康益处。尽管如此，关于定期运动引起的血浆蛋白质组变化的数据有限。在这里，我们在 654 名健康的人类研究参与者进行了大规模的血浆蛋白质组学分析，这些参与者在接受监督的 20 周耐力运动训练干预前后接受了分析。我们发现了数百种被调节的循环蛋白，其中许多是已知分泌的蛋白。我们强调了与血管生成、铁稳态和细胞外基质有关的蛋白质，其中许多是新的，包括训练诱导的成纤维细胞激活蛋白（FAP）增加，FAP 是一种与身体成分平衡有关的膜结合和循环蛋白。我们将蛋白质的变化与训练诱导的最大摄氧量适应相关联，并在一个外部运动队列中验证了我们的主要发现。此外，我们还表明，FAP 与 3 个独立的基于人群的队列中的存活率呈正相关。
