Sheward W J, Lutz E M, Harmar A J
MRC Brain Metabolism Unit, University Department of Pharmacology, Edinburgh, U.K.
Neuroscience. 1995 Jul;67(2):409-18. doi: 10.1016/0306-4522(95)00048-n.
The distribution of rat vasoactive intestinal peptide2 (VIP2) receptor messenger RNA in the brain and the pituitary gland was examined by in situ hybridization and by ribonuclease protection assay. labelled cells were found chiefly in the suprachiasmatic nucleus, the central nucleus of the amygdala and the thalamus (the lateral geniculate nucleus, and the paraventricular, mediodorsal and ventral nuclei of the thalamus). The distribution of the VIP2 receptor overlaps only in part with that of the VIP1 receptor, for example in the hippocampus, where VIP2 receptor messenger RNA was found in the pyramidal cells of the CA1-CA3 subfields and in the granule cells of the dentate gyrus. Small numbers of neurons containing high concentrations of VIP2 receptor messenger RNA were present in the brainstem in the principal sensory trigeminal nucleus and in the substantia gelatinosa of the spinal cord, suggesting a role for the VIP2 receptor in the processing of sensory information. The presence of the VIP2 receptor in the suprachiasmatic nucleus suggests that it is this receptor subtype which is involved in the control of circadian rhythms.
采用原位杂交和核糖核酸酶保护分析方法，研究了大鼠血管活性肠肽2（VIP2）受体信使核糖核酸在脑和垂体中的分布情况。标记细胞主要见于视交叉上核、杏仁核中央核和丘脑（外侧膝状体以及丘脑室旁核、背内侧核和腹侧核）。VIP2受体的分布仅部分与VIP1受体的分布重叠，例如在海马体中，在CA1 - CA3亚区的锥体细胞和齿状回的颗粒细胞中发现了VIP2受体信使核糖核酸。在脑干的主要感觉三叉神经核和脊髓的胶状质中存在少量含有高浓度VIP2受体信使核糖核酸的神经元，这表明VIP2受体在感觉信息处理中发挥作用。视交叉上核中VIP2受体的存在表明，正是这种受体亚型参与了昼夜节律的控制。
