Dussaillant M, Sarrieau A, Gozes I, Berod A, Rostene W
INSERM U339, Centre de Recherche Hôpital Saint-Antoine, Paris, France.
Neuroscience. 1992 Oct;50(3):519-30. doi: 10.1016/0306-4522(92)90444-7.
The distribution of cells expressing vasoactive intestinal peptide/peptide histidine isoleucine-amide precursor messenger RNA was investigated in the rat brain and pituitary by in situ hybridization using a synthetic 35S-labeled oligonucleotide probe. Detection of labeled neurons by light-microscopic radioautography revealed a selective repartition of the messenger RNA-expressing cells. Several major vasoactive intestinal peptide/peptide histidine isoleucine-amide messenger RNA-containing cell groups were demonstrated including layers II-VI of the cerebral cortex, the suprachiasmatic nucleus and various thalamic structures such as the ventrolateral, posterior, lateral reticular, paracentralis and gelatinosus nuclei. Positive cells, to a lesser extent, were also found in the limbic system, medial preoptic area, superior and inferior colliculi as well as in the central gray matter. They were totally absent in the pituitary and the pineal gland of normal rats. The results of the present study provide a detailed mapping of neurons expressing vasoactive intestinal peptide/peptide histidine isoleucine-amide messenger RNA in the adult rat brain. The predominance of vasoactive intestinal peptide/peptide histidine isoleucine-amide messenger RNA-containing neurons in the cerebral cortex, suprachiasmatic nucleus and thalamus suggest that vasoactive intestinal peptide is mainly involved in the control of cortical informations, circadian rhythms and sensory perception in agreement with several physiological data.
使用合成的35S标记寡核苷酸探针，通过原位杂交技术研究了大鼠脑和垂体中表达血管活性肠肽/肽组氨酸异亮氨酸酰胺前体信使核糖核酸的细胞分布。通过光学显微镜放射自显影检测标记神经元，揭示了表达信使核糖核酸的细胞的选择性重新分布。证实了几个主要的含血管活性肠肽/肽组氨酸异亮氨酸酰胺信使核糖核酸的细胞群，包括大脑皮层的II - VI层、视交叉上核以及各种丘脑结构，如腹外侧核、后核、外侧网状核、中央旁核和胶状核。在边缘系统、内侧视前区、上丘和下丘以及中央灰质中也发现了较少的阳性细胞。在正常大鼠的垂体和松果体中完全没有发现它们。本研究结果提供了成年大鼠脑中表达血管活性肠肽/肽组氨酸异亮氨酸酰胺信使核糖核酸的神经元的详细图谱。大脑皮层、视交叉上核和丘脑中含血管活性肠肽/肽组氨酸异亮氨酸酰胺信使核糖核酸的神经元占优势，这表明血管活性肠肽主要参与皮质信息控制、昼夜节律和感觉感知，这与一些生理学数据一致。
