Philpot B D, Klintsova A Y, Brunjes P C
Department of Psychology, University of Virginia, Charlottesville, 22903, USA.
Neuroscience. 1995 Aug;67(4):1009-19. doi: 10.1016/0306-4522(95)00095-z.
Immunocytochemistry was used to characterize oligodendrocyte maturation in the developing mammalian olfactory system. Postnatal day 10-16, 20, 30 and adult rats were examined, as well as postnatal day 20, 30, 40 and adult Monodelphis domestica (the grey, short-tailed opossum). In rats, oligodendrocyte/myelin-immunoreactivity first appears in the accessory olfactory bulb by day 11, with labeling rapidly increasing throughout the entire bulb over the next five days. An adult pattern of immunoreactivity, characterized by dense labeling in the granule cell layer, sparse immunoreactivity in the external plexiform layer, and staining along the periphery of glomeruli, is attained by day 30. Staining is apparent in both the lateral olfactory tract and anterior commissure by day 11, and becomes heavy by day 20. While patterns of oligodendrocyte/myelin-immunoreactivity in the adult Monodelphis and rat bulb are similar, staining first appears much later in the opossum (around day 30), and maturation occurs more slowly. For example, rostral-caudal gradients in the development of staining in the anterior commissure were noted which were not seen in the rat. These differences emerge because Monodelphis' slower growth allows more resolution into developmental sequences. Finally, in rats, unilateral naris closure on the day after birth, which significantly alters normal patterns of bulb development, has no effect on the pattern and level of immunoreactivity even after long (30 day) survival periods. In both normal and naris occluded rats, oligodendrocyte/myelin-immunoreactivity is found in caudal aspects of the rat bulb on day 11 and subsequently progresses throughout the entire bulb over the next five days. Patterns in the Monodelphis bulb mirror those observed in the rat, however, staining appears later and progresses more slowly, suggesting Monodelphis is a useful animal for examining early myelin formation.
免疫细胞化学被用于表征发育中的哺乳动物嗅觉系统中少突胶质细胞的成熟过程。研究了出生后第10 - 16天、20天、30天的大鼠以及成年大鼠，还有出生后第20天、30天、40天的家短尾负鼠（灰色短尾负鼠）和成年家短尾负鼠。在大鼠中，少突胶质细胞/髓磷脂免疫反应性在出生后第11天首次出现在副嗅球，在接下来的五天里，整个嗅球的标记迅速增加。到第30天达到成年免疫反应模式，其特征是颗粒细胞层标记密集，外丛状层免疫反应稀疏，以及沿肾小球周边染色。在出生后第11天，外侧嗅束和前连合中就有明显染色，到第20天变得浓重。虽然成年家短尾负鼠和大鼠嗅球中的少突胶质细胞/髓磷脂免疫反应性模式相似，但负鼠中的染色首次出现要晚得多（约在第30天），并且成熟过程更慢。例如，在前连合染色发育过程中观察到了头尾梯度，而在大鼠中未见到这种情况。这些差异的出现是因为家短尾负鼠生长较慢，使得发育序列的分辨率更高。最后，在大鼠中，出生后第二天单侧鼻孔闭合，这会显著改变嗅球发育的正常模式，但即使在长时间（30天）存活期后，对免疫反应性的模式和水平也没有影响。在正常和鼻孔闭塞的大鼠中，出生后第11天在大鼠嗅球的尾部发现少突胶质细胞/髓磷脂免疫反应性，随后在接下来的五天里在整个嗅球中发展。家短尾负鼠嗅球中的模式与大鼠中观察到的模式相似，然而，染色出现较晚且进展较慢，这表明家短尾负鼠是研究早期髓磷脂形成的有用动物。
