Philpot B D, Jazaeri A A, Brunjes P C
Department of Psychology, University of Virginia, Charlottesville 22903.
Brain Res Dev Brain Res. 1994 Feb 18;77(2):265-70. doi: 10.1016/0165-3806(94)90202-x.
The offspring of Monodelphis domestica (grey, short-tailed opossum) are born in a very immature state after a short (14-day) gestation period. As a result, they provide a useful mammalian model for examining quite early stages of brain maturation. The present study demonstrates that the onset and development of serotonergic (5-HT) projections to the Monodelphis olfactory bulb can be traced entirely after birth. 5-HT was visualized in tissue sections from postnatal day 5 (P5), P10, P20, P30, and adult opossums using avidin-biotin peroxidase immunocytochemistry. 5-HT afferent fibers are rare in the bulb until P10. Maturation occurs slowly, but by P30, 5-HT fibers assume the adult form characterized by preferential glomerular innervation. Due to the postnatal development, slow maturation, and specificity of innervation patterns, the Monodelphis bulb provides a model with which to study the regulation of serotonergic development.
家短尾负鼠（Monodelphis domestica）的后代在经历短暂的（14天）妊娠期后，出生时处于非常不成熟的状态。因此，它们为研究大脑成熟的相当早期阶段提供了一个有用的哺乳动物模型。本研究表明，向家短尾负鼠嗅球的5-羟色胺能（5-HT）投射的起始和发育完全可以在出生后追踪。使用抗生物素蛋白-生物素过氧化物酶免疫细胞化学方法，在出生后第5天（P5）、P10、P20、P30的组织切片以及成年负鼠的组织切片中观察到了5-HT。在P10之前，嗅球中的5-HT传入纤维很少。成熟过程缓慢，但到P30时，5-HT纤维呈现出以优先支配肾小球为特征的成年形式。由于出生后的发育、缓慢的成熟过程以及神经支配模式的特异性，家短尾负鼠的嗅球提供了一个研究5-羟色胺能发育调节的模型。
