Monteón V M, Guzmán-Bracho C, Floriani-Verdugo J, Ramos-Echevarría A, Velasco-Castrejón O, Reyes P A
Departamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez, México, D.F.
Salud Publica Mex. 1995 May-Jun;37(3):232-5.
American trypanosomiasis (Chagas' disease) is becoming a relatively common condition in North America. Diagnosis at the chronic stage depends on demonstration of specific antibodies in body fluids, since parasitologic or pathologic diagnosis is uncertain at this stage. Therefore, standardization of immunodiagnostic techniques is mandatory, and it depends on antigen quality. Locally prepared antigens and crude extracts obtained from Mexican isolates, -both from infected vector and human cases-were compared using three different immunodiagnostic assays-indirect immunofluorescence, hemagglutination and enzyme linked immunosorbent assay (ELISA)-at two different laboratories from the Instituto Nacional de Cardiología and the Instituto Nacional de Diagnóstico y Referencia Epidemiológicos. Concordance between laboratories reached a significant Kappa value (0.8) and sensitivity, specificity and predictive values of individual diagnostic assays were adequate to use these tests in clinical diagnoses. This is the first attempt to standardize immunodiagnostic techniques in Mexico.
美洲锥虫病（恰加斯病）在北美正逐渐成为一种相对常见的病症。慢性期的诊断依赖于体液中特异性抗体的检测，因为在此阶段寄生虫学或病理学诊断并不确定。因此，免疫诊断技术的标准化是必需的，而这取决于抗原质量。使用来自国立心脏病学研究所和国家诊断与流行病学参考研究所的两个不同实验室的三种不同免疫诊断检测方法——间接免疫荧光法、血凝试验和酶联免疫吸附测定（ELISA），对从墨西哥分离株（来自受感染的传播媒介和人类病例）获得的本地制备抗原和粗提物进行了比较。实验室间的一致性达到了显著的卡帕值（0.8），且各个诊断检测方法的敏感性、特异性和预测值足以用于临床诊断。这是墨西哥首次尝试对免疫诊断技术进行标准化。
