未离心处理的种马精液的冷冻保存能力及繁殖力结果
Freezability and fertility results with uncentrifuged stallion semen.
作者信息
Heiskanen M L, Hilden L, Hyyppä S, Kangasniemi A, Pirhonen A, Mäenpää P H
机构信息
Department of Biochemistry & Biotechnology, University of Kuopio, Ypäjä, Finland.
出版信息
Acta Vet Scand. 1994;35(4):377-82. doi: 10.1186/BF03548311.
The first (1 to 3) sperm-rich fractions of the ejaculate were collected from 4 stallions using an open-ended vagina. The volume of the collected fractions was 12 +/- 8 ml with a density of 475 +/- 200 million spermatozoa/ml. Before freezing, the semen was diluted with a skim-milk based extender 1:1 to 1:8 (volume of semen: volume of extender), depending on the initial sperm concentration to achieve a final concentration of 100 million/ml. The total number of spermatozoa in an insemination dose ranged from 0.7 to 1 billion spermatozoa. Within 12 h after ovulation, 48 mares were inseminated in 70 cycles. The total single-cycle pregnancy rate at day 21 was 24%, but varied from 10% to 33% per cycle among the stallions.
使用开口阴道从4匹种马收集射精的最初（1至3）富含精子的部分。收集部分的体积为12±8毫升，密度为4.75±2亿个精子/毫升。冷冻前，根据初始精子浓度，将精液用基于脱脂乳的稀释剂按1:1至1:8（精液体积：稀释剂体积）进行稀释，以达到最终浓度1亿个/毫升。输精剂量中的精子总数为0.7至10亿个精子。在排卵后12小时内，70个周期中对48匹母马进行了输精。第21天的单周期总妊娠率为24%，但各匹种马的每个周期妊娠率在10%至33%之间有所不同。
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