Katila T, Celebi M, Koskinen E
Faculty of Veterinary Medicine, University of Helsinki, Saari, Finland.
Acta Vet Scand. 1996;37(3):361-5. doi: 10.1186/BF03548102.
Thirty-four mares were inseminated with frozen semen from one stallion during 2 oestrous cycles, every 48 h until ovulation took place and within 12 h after ovulation. Semen was frozen using the Colorado method. The insemination dose was from 200 to 400 x 10(6) progressively motile spermatozoa. Ovaries were examined every 12 h to determine time of ovulation. Examination for pregnancy was carried out using ultrasonography, 15 days after ovulation. Thirty-five per cent of mares inseminated < 24 h and 23% of mares inseminated between 24-48 h before ovulation were pregnant (p = 0.388). The pregnancy rate in all mares inseminated before ovulation was 30%. In the mares inseminated within 12 h of ovulation, it was 18% (p = 0.253). Younger mares (aged 4-10 yr) had a higher pregnancy rate (59%) than older mares (aged 11-15 yr) (23%), but the difference was not statistically significant (p = 0.057).
在两个发情周期内，对34匹母马每隔48小时用一匹种公马的冷冻精液进行人工授精，直至排卵发生，并在排卵后12小时内进行。精液采用科罗拉多方法冷冻。授精剂量为200至400×10⁶条进行性运动精子。每12小时检查一次卵巢以确定排卵时间。排卵后15天使用超声检查妊娠情况。在排卵前<24小时进行人工授精的母马中有35%怀孕，在排卵前24 - 48小时进行人工授精的母马中有23%怀孕（p = 0.388）。所有在排卵前进行人工授精的母马的妊娠率为30%。在排卵后12小时内进行人工授精的母马中，妊娠率为18%（p = 0.253）。年轻母马（4 - 10岁）的妊娠率（59%）高于年长母马（11 - 15岁）（23%），但差异无统计学意义（p = 0.057）。
