Koskinen E, Lindeberg H, Kuntsi H, Ruotsalainen L, Katila T
Agricultural Research Centre, Equine Research Station, Ypäjä, Finland.
Zentralbl Veterinarmed A. 1990 Feb;37(1):77-80. doi: 10.1111/j.1439-0442.1990.tb00878.x.
In a first experiment, 11 Finnhorse mares were examined every six hours during late oestrus by rectal palpation and ultrasonography to determine the time of ovulation. The mares were inseminated over one to three subsequent cycles, 6-12 (n = 5), 12-18 (n = 5), 18-24 (n = 5) and 24-30 (n = 5) hours after ovulation. Pregnancies were terminated by prostaglandin injection 21 days after insemination. All mares inseminated within 18 hours of ovulation conceived but no mare inseminated 24 hours or more after ovulation conceived. In a second experiment, 14 mares were examined every day at about the same time and inseminated after ovulation had been detected, within 0-27 hours of ovulation. Pregnancies were terminated by prostaglandin injection 25 days after insemination and the animals inseminated again. Forty-six per cent of the mares (13 out of 28) were pregnant 16 days after insemination but there was early embryonic death in five mares, i.e. only 29% of the mares became and remained pregnant. The equine ovum seems to be capable of being fertilized for at least 18 hours after ovulation. However, the high incidence of early embryonic death in the second experiment suggests that the viability of the embryo may have decreased.
在第一个实验中，在发情后期，每隔6小时通过直肠触诊和超声检查对11匹芬兰马母马进行检查，以确定排卵时间。在随后的一至三个周期中，在排卵后6 - 12小时（n = 5）、12 - 18小时（n = 5）、18 - 24小时（n = 5）和24 - 30小时（n = 5）对母马进行授精。授精21天后，通过注射前列腺素终止妊娠。所有在排卵后18小时内授精的母马都受孕了，但排卵后24小时或更长时间授精的母马没有受孕。在第二个实验中，每天大约在同一时间对14匹母马进行检查，在检测到排卵后，在排卵后0 - 27小时内进行授精。授精25天后，通过注射前列腺素终止妊娠，然后再次对动物进行授精。授精16天后，46%的母马（28匹中的13匹）怀孕，但有5匹母马发生早期胚胎死亡，即只有29%的母马成功受孕并维持妊娠。马的卵子似乎在排卵后至少18小时内能够受精。然而，第二个实验中早期胚胎死亡的高发生率表明胚胎活力可能已经下降。
