Shroyer K R, Brookes C G, Markham N E, Shroyer A L
Department of Pathology, University of Colorado Health Sciences Center, Denver, USA.
Am J Clin Pathol. 1995 Sep;104(3):299-305. doi: 10.1093/ajcp/104.3.299.
Polymerase chain reaction (PCR) and in situ hybridization were used to test for the presence of human papillomavirus (HPV) DNA in cases of anorectal squamous cell carcinoma. Human papillomavirus was detected by PCR with L1 consensus sequence primers in 22 of 27 cases, including 10 of 11 cases with a prominent basaloid pattern and 12 of 16 cases without basaloid patterns of differentiation. Slot blot hybridization identified HPV type 16 as the most common type, present in 7 of 10 cases of basaloid carcinoma and 10 of 12 cases without basaloid features. In situ hybridization confirmed the presence of HPV in tumor cell nuclei of five cases of basaloid carcinoma and in eight cases of squamous cell carcinoma without basaloid pattern. The authors conclude that the prevalence of HPV in cases of anorectal squamous cell carcinoma is unrelated to the presence or absence of a basaloid pattern of differentiation.
采用聚合酶链反应（PCR）和原位杂交技术检测肛管直肠鳞状细胞癌病例中人类乳头瘤病毒（HPV）DNA的存在情况。使用L1共有序列引物通过PCR在27例中的22例检测到HPV，其中包括11例具有显著基底样模式的病例中的10例以及16例无基底样分化模式的病例中的12例。狭缝印迹杂交确定HPV 16型为最常见类型，存在于10例基底样癌中的7例以及12例无基底样特征的病例中的10例。原位杂交证实5例基底样癌和8例无基底样模式的鳞状细胞癌的肿瘤细胞核中存在HPV。作者得出结论，肛管直肠鳞状细胞癌病例中HPV的流行率与是否存在基底样分化模式无关。
