Shroyer K R, Kim J G, Manos M M, Greer C E, Pearlman N W, Franklin W A
Department of Pathology, University of Colorado Health Sciences Center, Denver.
Arch Surg. 1992 Jun;127(6):741-4. doi: 10.1001/archsurg.1992.01420060121018.
We used polymerase chain reaction DNA amplification methods for the detection and typing of genital human papillomaviruses in paraffin-embedded tissue sections of five patients with anorectal squamous cell carcinoma and 22 patients with colonic adenocarcinoma. The cases were further tested by in situ hybridization with biotin-labeled probes specific for human papillomavirus types 6/11, 16/18, and 31/33/35. By polymerase chain reaction, human papillomavirus DNA was demonstrated in all of the cases of anorectal squamous cell carcinoma and in none of the cases of colonic adenocarcinoma for which analyzable DNA was available. Tumor cell nuclei stained for human papillomavirus DNA by in situ hybridization in four of the five cases of squamous cell carcinoma and in none of the cases of colonic adenocarcinoma. We conclude that human papillomavirus types usually associated with malignant transformation are uniformly present in anorectal squamous cell carcinoma but are absent from adenocarcinoma of the colon.
我们采用聚合酶链反应DNA扩增方法，对5例肛管鳞状细胞癌患者和22例结肠腺癌患者石蜡包埋组织切片中的生殖道人乳头瘤病毒进行检测和分型。通过用生物素标记的、针对人乳头瘤病毒6/11型、16/18型和31/33/35型的探针进行原位杂交，对这些病例作进一步检测。通过聚合酶链反应，在所有可分析DNA的肛管鳞状细胞癌病例中均检测到了人乳头瘤病毒DNA，而在所有结肠腺癌病例中均未检测到。在5例鳞状细胞癌病例中的4例，肿瘤细胞核经原位杂交显示人乳头瘤病毒DNA呈阳性，而所有结肠腺癌病例均未显示阳性。我们得出结论，通常与恶性转化相关的人乳头瘤病毒类型在肛管鳞状细胞癌中普遍存在，而在结肠腺癌中则不存在。
