Mendonca S C, De Luca P M, Mayrink W, Restom T G, Conceicao-Silva F, Da-Cruz A M, Bertho A L, Da Costa C A, Genaro O, Toledo V P
Departamento de Protozoologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
Am J Trop Med Hyg. 1995 Aug;53(2):195-201. doi: 10.4269/ajtmh.1995.53.195.
Forty-three Brazilians were immunized against American tegumentary leishmaniasis using a vaccine made of whole antigens from killed promastigotes of five American dermotropic Leishmania strains. None of the immunized subjects had a positive reaction in the Montenegro skin test (leishmanin) before vaccination, and 74% developed positive reactions in the skin test after vaccination. The proliferative responses of peripheral blood mononuclear cells (PBMC) induced by antigens from dermotropic Leishmania species were significantly higher after vaccination than before vaccination. However, with antigens from L. chagasi (a causative agent of American visceral leishmaniasis), there was no significant difference between the proliferative responses obtained before and after vaccination. Interferon-gamma was detected in the supernatants of L. braziliensis antigen-stimulated PBMC cultures after vaccination (but not before vaccination). One year after vaccination, PBMC were obtained from eight of the immunized individuals and stimulated with L. braziliensis antigens in proliferative response assays. In all cases, the majority of the responding cells were CD8+ T cells, in contrast to the results of a group of patients with active lesions of tegumentary leishmaniasis, whose L. braziliensis-reactive cells were mainly of the CD4+ T cell phenotype.
43名巴西人使用由5种美洲皮肤利什曼原虫株的死前鞭毛体全抗原制成的疫苗，接种预防美洲皮肤利什曼病。所有接种者在接种前的蒙氏皮肤试验（利什曼菌素）中均无阳性反应，74%的接种者在接种后皮肤试验呈阳性反应。接种后，皮肤利什曼原虫种抗原诱导的外周血单个核细胞（PBMC）增殖反应显著高于接种前。然而，对于来自恰加斯利什曼原虫（美洲内脏利什曼病病原体）的抗原，接种前后获得的增殖反应无显著差异。接种后（而非接种前），在巴西利什曼原虫抗原刺激的PBMC培养上清液中检测到γ干扰素。接种一年后，从8名接种个体中获取PBMC，并在增殖反应试验中用巴西利什曼原虫抗原刺激。在所有病例中，大多数反应细胞为CD8 + T细胞，这与一组有皮肤利什曼病活动性病变患者的结果相反，后者对巴西利什曼原虫反应的细胞主要为CD4 + T细胞表型。
