三种杂二糖（4-O-β-D-吡喃葡萄糖基-6-脱氧-D-葡萄糖、4-O-β-D-吡喃葡萄糖基-D-甘露糖胺和4-O-β-D-吡喃葡萄糖基-D-甘露糖）的合成及其结构通过碳-13核磁共振和质谱分析得以确证。
Synthesis of three hetero disaccharides, 4-O-beta-glucopyranosyl-6-deoxy-D-glucose, 4-O-beta-D-glucopyranosyl-D-mannosamine, and 4-O-beta-D-glucopyranosyl-D-mannose, and confirmation of their structures by C-13 NMR and MS.
作者信息
Tariq M A, Hayashi K
机构信息
National Food Research Institute, Ibaraki, Japan.
出版信息
Biochem Biophys Res Commun. 1995 Sep 14;214(2):568-75. doi: 10.1006/bbrc.1995.2323.
The three disaccharides, 4-O-beta-D-glucopyranosyl-6-deoxy-D-glucose (Glc-6-deoxy-Glc), 4-O-beta-D-glucopyranosyl-D-mannosamine (Glc-ManN), 4-O-beta-D-glucopyranosyl-D-mannose (Glc-Man), were synthesized from equimolar amounts of 6-deoxy-glucose and alpha-D-glucose-1-phosphate (G-1-P), D-mannosamine and alpha-D-glucose-1-phosphate (G-1-P), and D-mannose and alpha-D-glucose-1-phosphate (G-1-P), respectively, using cellobiose phosphorylase from Cellvibrio gilvus. The yields were 60%, 10% and 50% based on the initial amounts of 6-deoxy-D-glucose, D-mannosamine and D-mannose, respectively. The structures of disaccharides were confirmed by NMR and mass spectroscopy analysis.
三种二糖，4-O-β-D-吡喃葡萄糖基-6-脱氧-D-葡萄糖（Glc-6-脱氧-Glc）、4-O-β-D-吡喃葡萄糖基-D-甘露糖胺（Glc-ManN）、4-O-β-D-吡喃葡萄糖基-D-甘露糖（Glc-Man），分别由等摩尔量的6-脱氧葡萄糖与α-D-葡萄糖-1-磷酸（G-1-P）、D-甘露糖胺与α-D-葡萄糖-1-磷酸（G-1-P）、D-甘露糖与α-D-葡萄糖-1-磷酸（G-1-P），使用来自吉尔维氏纤维弧菌的纤维二糖磷酸化酶合成。基于6-脱氧-D-葡萄糖、D-甘露糖胺和D-甘露糖的初始量，产率分别为60%、10%和50%。二糖的结构通过核磁共振和质谱分析得以确认。
Zotero 插件