Dobson S J, Colwell R R, McMeekin T A, Franzmann P D
Department of Agricultural Science, University of Tasmania, Australia.
Int J Syst Bacteriol. 1993 Jan;43(1):77-83. doi: 10.1099/00207713-43-1-77.
Phenotypic data and phospholipid ester-linked fatty acid profiles indicate that pigmented bacterial strains isolated from a hypersaline Antarctic lake are members of the "flavobacterium-bacteroides" phylum and may represent new taxa. Nearly complete 16S rRNA sequences were obtained for representative strains by directly sequencing the polymerase chain reaction-amplified 16S rRNA gene. Sequence signatures confirmed that these organisms were members of the flavobacterium-bacteroides phylum. A phylogenetic analysis, in which the sequences of the Antarctic strains were compared with a large number of sequences available for members of the flavobacterium-bacteroides phylum, showed that the Antarctic strains were phylogenetically distinct. The new species cluster with a group of organisms that contains the type species of the genus Flavobacterium, Flavobacterium aquatile. Two new species are described, for which the names Flavobacterium gondwanense and Flavobacterium salegens are proposed; strains ACAM 44 (= DSM 5423) and ACAM 48 (= DSM 5424) are the type strains of F. gondwanense and F. salegens, respectively.
表型数据和磷脂酯连接脂肪酸谱表明，从南极高盐湖泊中分离出的色素细菌菌株属于“黄杆菌 - 拟杆菌”门，可能代表新的分类单元。通过对聚合酶链反应扩增的16S rRNA基因进行直接测序，获得了代表性菌株的近乎完整的16S rRNA序列。序列特征证实这些生物是黄杆菌 - 拟杆菌门的成员。系统发育分析将南极菌株的序列与大量黄杆菌 - 拟杆菌门成员的可用序列进行比较，结果表明南极菌株在系统发育上是不同的。新物种与一组包含黄杆菌属模式种水生黄杆菌的生物聚类。描述了两个新物种，分别命名为冈瓦纳黄杆菌（Flavobacterium gondwanense）和盐生黄杆菌（Flavobacterium salegens）；菌株ACAM 44（= DSM 5423）和ACAM 48（= DSM 5424）分别是冈瓦纳黄杆菌和盐生黄杆菌的模式菌株。
