Das L, Das S K, Chu E H, Sinsheimer J E
Department of Human Genetics, University of Michigan, Ann Arbor 48109.
Mutat Res. 1993 Mar;299(1):19-24. doi: 10.1016/0165-1218(93)90114-s.
Mouse lymphocytes in vivo or in vitro were exposed for 24 h to 4 aliphatic epoxides, glycidyl 1-naphthyl ether, glycidyl 4-nitrophenyl ether, 1-naphthyl-propylene oxide and trichloropropylene oxide (TCPO), and tested for the induction of chromosomal aberrations (CA). These epoxides were among the most genotoxic aliphatic epoxides in our previous studies. With the exception of TCPO, the test epoxides caused significant increases in CA in vivo compared to a negative control. There were concentration related increases in CA for all 4 epoxides in vitro and TCPO produced the greatest cellular toxicity and genotoxic effects towards cultured lymphocytes. The difference in the order of genotoxicity for the two test systems can be explained on the basis of a much shorter half-life for TCPO than for the other epoxides.
将小鼠淋巴细胞在体内或体外暴露于4种脂肪族环氧化物、缩水甘油基1-萘基醚、缩水甘油基4-硝基苯基醚、1-萘基环氧丙烷和三氯环氧丙烷（TCPO）24小时，然后检测其对染色体畸变（CA）的诱导作用。在我们之前的研究中，这些环氧化物属于最具遗传毒性的脂肪族环氧化物。除TCPO外，与阴性对照相比，受试环氧化物在体内均引起CA显著增加。在体外，所有4种环氧化物的CA均呈浓度依赖性增加，且TCPO对培养淋巴细胞产生的细胞毒性和遗传毒性作用最大。两种测试系统中遗传毒性顺序的差异可以基于TCPO的半衰期比其他环氧化物短得多来解释。
