Ruzdijic S, Pekovic S, Kanazir S, Ivkovic S, Stojiljkovic M, Rakic L
Department of Neurobiology and Immunology, University of Belgrade, Yugoslavia.
Brain Res. 1993 Jan 22;601(1-2):230-40. doi: 10.1016/0006-8993(93)91715-5.
The expression of the proto-oncogene c-fos is increased in neuronal cells by a number of stimuli and the usefulness of this gene as a marker of neuronal activity has been demonstrated. The temporal and spatial expression of c-fos mRNA following the induction of a unilateral cortical lesion have been investigated in the rat brain by Northern blot analysis and in situ hybridization histochemistry. It was observed that the lesion evoked a rapid increase (20-fold) in the content of c-fos mRNA in the ipsilateral cortex, whereas in the contralateral cortex c-fos mRNA expression was more modest (7-fold). In the whole hippocampus a large and very rapid increase (17-fold) of c-fos mRNA expression was detected. The effect of a cortical lesion on Ca2+ uptake and membrane potential was also investigated. Using synaptosomes as a model system, we have provided evidence that Ca2+ entry via membrane depolarization increases in coordination with c-fos gene expression in neuronal cells. The principal conclusions from this study are that cortical lesions induce transient expression of the c-fos gene in specific neuronal cells of the rat brain.
原癌基因c-fos在神经元细胞中会因多种刺激而表达增加，并且该基因作为神经元活动标志物的实用性已得到证实。通过Northern印迹分析和原位杂交组织化学方法，研究了大鼠脑单侧皮质损伤诱导后c-fos mRNA的时空表达。观察到损伤引起同侧皮质中c-fos mRNA含量迅速增加（20倍），而对侧皮质中c-fos mRNA表达增加幅度较小（7倍）。在整个海马体中，检测到c-fos mRNA表达大幅且迅速增加（17倍）。还研究了皮质损伤对Ca2+摄取和膜电位的影响。以突触体作为模型系统，我们提供了证据表明，通过膜去极化的Ca2+内流与神经元细胞中c-fos基因表达协同增加。这项研究的主要结论是，皮质损伤在大鼠脑特定神经元细胞中诱导c-fos基因的瞬时表达。
