Asanuma M, Ogawa N, Hirata H, Chou H, Tanaka K, Mori A
Department of Neuroscience, Okayama University Medical School, Japan.
J Neural Transm Gen Sect. 1992;90(3):163-9. doi: 10.1007/BF01250958.
The rough handling with repeated saline administration (1.2 ml/kg s.c. for 7 days) enhanced cortical c-fos mRNA expression in the rat brain after a single saline stimulation (1.2 ml/kg s.c.) due to increasing baseline c-fos mRNA levels, whereas the gentle handling with repeated saline administration declined c-fos mRNA expression after a single injection due to decreasing the baseline of c-fos mRNA levels. These two types of handling with the repeated injection led to diametrically opposite results on c-fos mRNA expression after a single stimulation. Neither two types of handling with repeated saline injections affected the net increment of c-fos mRNA induction after a single stimulation, therefore, the effects of handling with repeated treatment on c-fos mRNA expression might be independent of the effects of a single saline stimulation. The present study suggests that c-fos mRNA induction after a single stimulation might be affected by the types or intensities of handling and that care must be taken to estimate c-fos mRNA induction.
反复腹腔注射生理盐水（1.2 ml/kg，皮下注射，共7天）的粗暴操作，由于增加了c-fos mRNA的基线水平，在单次生理盐水刺激（1.2 ml/kg，皮下注射）后增强了大鼠脑皮质c-fos mRNA的表达；而反复腹腔注射生理盐水的轻柔操作，由于降低了c-fos mRNA的基线水平，在单次注射后降低了c-fos mRNA的表达。这两种反复注射的操作方式，在单次刺激后对c-fos mRNA的表达产生了截然相反的结果。两种反复腹腔注射生理盐水的操作方式均未影响单次刺激后c-fos mRNA诱导的净增量，因此，反复处理对c-fos mRNA表达的影响可能独立于单次生理盐水刺激的影响。本研究表明，单次刺激后c-fos mRNA的诱导可能受操作方式或强度的影响，在评估c-fos mRNA诱导时必须谨慎。
