Aylward R L, Totterdell S
University Department of Pharmacology, Oxford, Great Britain.
J Chem Neuroanat. 1993 Jan-Feb;6(1):31-42. doi: 10.1016/0891-0618(93)90005-o.
Neurons in the hippocampus, amygdala and entorhinal cortex which project to the nucleus accumbens were labelled retrogradely following injection of horseradish peroxidase. The injections were targetted on the medial part of the nucleus accumbens, but some injection sites included the whole nucleus. Projection neurons in all three areas were found to be spiny, and from the entorhinal cortex and ventral subiculum of the hippocampus they were pyramidal neurons. Somatostatin (S28(1-12)-immunoreactive neurons were found in all parts of the three limbic areas examined. They were found to have various morphologies, but in the electron microscope all had the ultrastructural characteristics of interneurons. In the hippocampus the stratum lacunosum was found to contain the most immunoreactive fibres while most cells lay in the stratum oriens. In the amygdala the densest staining for both cells and fibres was in the central nucleus. In the entorhinal cortex somatostatin-immunoreactive fibres and cells seemed to have no preferential distribution. Examination of somatostatin-immunoreactive profiles in the electron microscope revealed that the majority of synaptic contacts were made with dendrites, many of which were spine-bearing. In the light microscope somatostatin-immunoreactive fibres could be seen to lie near the somata and proximal dendrites of neurons that projected to the nucleus accumbens. In the electron microscope it was found that somatostatin-immunoreactive boutons were in symmetrical synaptic contact with the somata and proximal dendrites of neurons in the ventral subiculum, entorhinal cortex and amygdala which project to the nucleus accumbens.
在向伏隔核注射辣根过氧化物酶后，对海马体、杏仁核和内嗅皮质中投射至伏隔核的神经元进行了逆行标记。注射部位靶向伏隔核的内侧部分，但有些注射位点包括了整个核。发现这三个区域的投射神经元都有棘，并且在内嗅皮质和海马体腹侧下托中它们是锥体神经元。在所检查的三个边缘区域的所有部位都发现了生长抑素（S28(1 - 12)）免疫反应性神经元。它们具有各种形态，但在电子显微镜下都具有中间神经元的超微结构特征。在海马体中，腔隙层含有最多的免疫反应性纤维，而大多数细胞位于原层。在杏仁核中，细胞和纤维的染色最密集的是中央核。在内嗅皮质中，生长抑素免疫反应性纤维和细胞似乎没有优先分布。在电子显微镜下对生长抑素免疫反应性轮廓的检查显示，大多数突触接触是与树突形成的，其中许多树突带有棘。在光学显微镜下，可以看到生长抑素免疫反应性纤维位于投射至伏隔核的神经元的胞体和近端树突附近。在电子显微镜下发现，生长抑素免疫反应性终扣与投射至伏隔核的腹侧下托、内嗅皮质和杏仁核中神经元的胞体和近端树突形成对称突触接触。
