支气管肺泡灌洗术中上皮细胞的鉴定

Identification of epithelial cells in bronchoalveolar lavage.

Finotto S, Rado V, Dal Vecchio A, Milani G, Fabbri L M, Maestrelli P

Institute of Occupational Medicine, University of Padova, Italy.

Hum Exp Toxicol. 1993 Jan;12(1):43-6. doi: 10.1177/096032719301200109.

Abstract

Damage to the bronchial epithelium occurs after the inhalation of toxic substances and allergens, and through virus infections and it may lead to increased desquamation of epithelial cells in bronchoalveolar lavage (BAL). 2. In this study we compared two methods of staining the epithelial cells of BAL, the conventional cytochemical May Grunwald-Giemsa stain (MGG) and an immunocytochemical technique using a monoclonal antibody anti-human cytokeratin (CK) detected with APAAP immuno-alkaline phosphatase. BAL was obtained from 13 subjects and the epithelial cells were cytocentrifuged either immediately after collection (fraction A) or after washing (fraction B). 3. Higher percentages of epithelial cells were identified in fraction A with CK (20.0 +/- 5.1%) than in fraction A with MGG (11.2 +/- 2.3%), which recognized only ciliated epithelial cells. In fact a proportion of CK-positive cells (34%) in fraction A were not ciliated. Underestimation of epithelial cells by MGG compared to CK was more pronounced in fraction B (8.0 +/- 2.9% and 22.9 +/- 3.0%, respectively) as there was a relative loss of ciliated CK+ cells after washings. 4. These results suggest that immunocytochemical staining with an anti-cytokeratin monoclonal antibody is more sensitive than using the MGG stain in detecting epithelial cells in BAL.

摘要

吸入有毒物质和过敏原后，以及通过病毒感染，支气管上皮会受到损伤，这可能导致支气管肺泡灌洗（BAL）中上皮细胞的脱落增加。2. 在本研究中，我们比较了两种对BAL上皮细胞进行染色的方法，传统的细胞化学方法美蓝-姬姆萨染色（MGG）和一种免疫细胞化学技术，该技术使用抗人细胞角蛋白（CK）单克隆抗体，通过碱性磷酸酶抗碱性磷酸酶（APAAP）免疫检测。从13名受试者中获取BAL，上皮细胞在收集后立即进行细胞离心涂片（A部分）或洗涤后进行细胞离心涂片（B部分）。3. A部分中，使用CK检测到的上皮细胞百分比（20.0±5.1%）高于使用MGG检测到的（11.2±2.3%），MGG只能识别纤毛上皮细胞。实际上，A部分中一定比例（34%）的CK阳性细胞并非纤毛细胞。与CK相比，MGG对B部分上皮细胞的低估更为明显（分别为8.0±2.9%和22.9±3.0%），因为洗涤后纤毛CK+细胞相对减少。4. 这些结果表明，在检测BAL中的上皮细胞时，使用抗细胞角蛋白单克隆抗体进行免疫细胞化学染色比使用MGG染色更敏感。