吖啶橙、革兰氏和迈-格-姬姆萨染色法用于支气管肺泡灌洗液体细胞内生物计数的检测特征。
Test characteristics of acridine orange, Gram, and May-Grünwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid.
作者信息
De Brauwer E, Jacobs J, Nieman F, Bruggeman C, Drent M
机构信息
Department of Medical Microbiology, University Hospital Maastricht, The Netherlands.
出版信息
J Clin Microbiol. 1999 Feb;37(2):427-9. doi: 10.1128/JCM.37.2.427-429.1999.
Abstract
For enumeration of intracellular organisms (ICO) in bronchoalveolar lavage fluid samples, the May-Grünwald-Giemsa (MGG) stain displayed higher interobserver agreement than the acridine orange and Gram stains. The MGG stain offered a reliable enumeration of ICO when 200 cells were counted by one observer.
摘要
对于支气管肺泡灌洗液体样本中细胞内生物体（ICO）的计数，与吖啶橙染色和革兰氏染色相比，改良美蓝-姬姆萨（MGG）染色显示出更高的观察者间一致性。当由一名观察者计数200个细胞时，MGG染色能对ICO进行可靠计数。
相似文献
1
Test characteristics of acridine orange, Gram, and May-Grünwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid.吖啶橙、革兰氏和迈-格-姬姆萨染色法用于支气管肺泡灌洗液体细胞内生物计数的检测特征。
J Clin Microbiol. 1999 Feb;37(2):427-9. doi: 10.1128/JCM.37.2.427-429.1999.
2
Gram stain of bronchoalveolar lavage fluid in the early diagnosis of ventilator-associated pneumonia.支气管肺泡灌洗液体的革兰氏染色在呼吸机相关性肺炎早期诊断中的应用
Br J Anaesth. 1999 Dec;83(6):845-9. doi: 10.1093/bja/83.6.845.
3
Comparison of acridine orange stain with culture and gram stain of needle aspirate in experimental Pseudomonas pneumonia.吖啶橙染色与实验性铜绿假单胞菌肺炎针吸物培养及革兰氏染色的比较
Am Rev Respir Dis. 1991 Oct;144(4):959-61. doi: 10.1164/ajrccm/144.4.959.
4
[Technical recommendations and best practice guidelines for May-Grünwald-Giemsa staining: literature review and insights from the quality assurance].[May-Grünwald-Giemsa染色的技术建议和最佳实践指南：文献综述与质量保证见解]
Ann Pathol. 2015 Aug;35(4):294-305. doi: 10.1016/j.annpat.2015.05.019. Epub 2015 Jul 16.
5
Comparison of acridine orange and Gram stains in bacterial keratitis.吖啶橙染色与革兰氏染色在细菌性角膜炎中的比较。
Am J Ophthalmol. 1988 Dec 15;106(6):735-7. doi: 10.1016/0002-9394(88)90710-6.
6
Legionella wadsworthii pneumonia: gram stain usefulness.
Med Mal Infect. 2011 Dec;41(12):669-70. doi: 10.1016/j.medmal.2011.09.015. Epub 2011 Oct 28.
7
Protected bronchoalveolar lavage in the diagnosis of ventilator-associated pneumonia.保护性支气管肺泡灌洗在呼吸机相关性肺炎诊断中的应用
Eur Respir J. 1996 Jul;9(7):1500-7. doi: 10.1183/09031936.96.09071500.
8
Identification of epithelial cells in bronchoalveolar lavage.支气管肺泡灌洗术中上皮细胞的鉴定
Hum Exp Toxicol. 1993 Jan;12(1):43-6. doi: 10.1177/096032719301200109.
9
Acridine orange and Gram stains in infectious keratitis.感染性角膜炎中的吖啶橙染色和革兰氏染色
Cornea. 1990 Apr;9(2):122-4.
10
Usefulness of microscopic examination of intracellular organisms in lavage fluid in ventilator-associated pneumonia.支气管肺泡灌洗液中细胞内微生物的显微镜检查在呼吸机相关性肺炎中的应用价值
Chest. 1994 Sep;106(3):889-94. doi: 10.1378/chest.106.3.889.
引用本文的文献
1
Soluble tissue factor generated by necroptosis-triggered shedding is responsible for thrombosis.坏死性凋亡引发的脱落所产生的可溶性组织因子是血栓形成的原因。
Cell Res. 2025 Sep 12. doi: 10.1038/s41422-025-01167-8.
2
Detection of Streptococcus pyogenes in an atypical hematological diagnostic case.非典型血液学诊断病例中化脓性链球菌的检测
Infection. 2024 Dec;52(6):2553-2554. doi: 10.1007/s15010-024-02335-5. Epub 2024 Jul 25.
3
Diagnosis of Septic Body Cavity Effusion in Dogs and Cats: Cytology vs. Bacterial Culture.犬猫脓毒性体腔积液的诊断：细胞学检查与细菌培养
Animals (Basel). 2024 Jun 11;14(12):1762. doi: 10.3390/ani14121762.
4
Cellular Localization of Selected Porphyrins and Their Effect on the In Vitro Motility of Human Colon Tumors and Normal Cells.选定卟啉类化合物的细胞定位及其对人结肠肿瘤和正常细胞体外运动性的影响。
Molecules. 2023 Mar 23;28(7):2907. doi: 10.3390/molecules28072907.
5
Influence of antibiotic therapy on the cytological diagnosis of ventilator-associated pneumonia.抗生素治疗对呼吸机相关性肺炎细胞学诊断的影响。
Intensive Care Med. 2008 May;34(5):865-72. doi: 10.1007/s00134-008-1015-x. Epub 2008 Feb 5.
6
Impact of ozone exposure on the phagocytic activity of human surfactant protein A (SP-A) and SP-A variants.臭氧暴露对人表面活性蛋白A（SP-A）及其变体吞噬活性的影响。
Am J Physiol Lung Cell Mol Physiol. 2008 Jan;294(1):L121-30. doi: 10.1152/ajplung.00288.2007. Epub 2007 Nov 2.
7
Effect of cysteine 85 on biochemical properties and biological function of human surfactant protein A variants.半胱氨酸85对人表面活性蛋白A变体生化特性及生物学功能的影响。
Biochemistry. 2007 Jul 17;46(28):8425-35. doi: 10.1021/bi7004569. Epub 2007 Jun 20.
8
Surfactant protein A2 (SP-A2) variants expressed in CHO cells stimulate phagocytosis of Pseudomonas aeruginosa more than do SP-A1 variants.在CHO细胞中表达的表面活性蛋白A2（SP-A2）变体比SP-A1变体更能刺激铜绿假单胞菌的吞噬作用。
Infect Immun. 2007 Mar;75(3):1403-12. doi: 10.1128/IAI.01341-06. Epub 2007 Jan 12.
9
Variation of infected cell count in bronchoalveolar lavage and timing of ventilator-associated pneumonia.支气管肺泡灌洗中感染细胞计数的变化与呼吸机相关性肺炎的发生时间
Intensive Care Med. 2004 Aug;30(8):1557-63. doi: 10.1007/s00134-004-2323-4. Epub 2004 May 13.
本文引用的文献
1
Early diagnosis of ventilator-associated pneumonia. Is it possible to define a cutoff value of infected cells in BAL fluid?呼吸机相关性肺炎的早期诊断。是否有可能确定支气管肺泡灌洗液中感染细胞的临界值？
Chest. 1996 Dec;110(6):1558-65. doi: 10.1378/chest.110.6.1558.
2
The application of generalizability theory to reliability assessment: an illustration using isometric force measurements.概化理论在可靠性评估中的应用：以等长肌力测量为例
Phys Ther. 1993 Jun;73(6):386-95; discussion 396-401. doi: 10.1093/ptj/73.6.386.
3
The effect of antibiotic therapy on recovery of intracellular bacteria from bronchoalveolar lavage in suspected ventilator-associated nosocomial pneumonia.抗生素治疗对疑似呼吸机相关性医院获得性肺炎患者支气管肺泡灌洗中细胞内细菌清除的影响
Chest. 1993 Feb;103(2):541-6. doi: 10.1378/chest.103.2.541.
4
Usefulness of microscopic examination of intracellular organisms in lavage fluid in ventilator-associated pneumonia.支气管肺泡灌洗液中细胞内微生物的显微镜检查在呼吸机相关性肺炎中的应用价值
Chest. 1994 Sep;106(3):889-94. doi: 10.1378/chest.106.3.889.
5
Role of bronchoalveolar lavage in mechanically ventilated patients with suspected pneumonia.
Eur J Clin Microbiol Infect Dis. 1994 Jul;13(7):549-58. doi: 10.1007/BF01971305.
6
Diagnosis of nosocomial bacterial pneumonia in intubated patients undergoing ventilation: comparison of the usefulness of bronchoalveolar lavage and the protected specimen brush.机械通气插管患者医院获得性细菌性肺炎的诊断：支气管肺泡灌洗与防污染样本毛刷的效用比较
Am J Med. 1988 Oct;85(4):499-506. doi: 10.1016/s0002-9343(88)80085-8.
7
[Broncho-alveolar lavage fluid technique at a central pathologic anatomy and cytology laboratory].[中央病理解剖学与细胞学实验室的支气管肺泡灌洗术]
Ann Pathol. 1990;10(4):278-81.
8
Diagnosis of ventilator-associated pneumonia by bacteriologic analysis of bronchoscopic and nonbronchoscopic "blind" bronchoalveolar lavage fluid.通过支气管镜和非支气管镜“盲法”支气管肺泡灌洗液体的细菌学分析诊断呼吸机相关性肺炎。
Am Rev Respir Dis. 1991 May;143(5 Pt 1):1121-9. doi: 10.1164/ajrccm/143.5_Pt_1.1121.
9
Management of bacterial pneumonia in ventilated patients. Protected bronchoalveolar lavage as a diagnostic tool.机械通气患者细菌性肺炎的管理。保护性支气管肺泡灌洗作为一种诊断工具。
Chest. 1992 Feb;101(2):500-8. doi: 10.1378/chest.101.2.500.
Zotero 插件