Baudoin C, Meneguzzi G, Portier M M, Demarchez M, Bernerd F, Pisani A, Ortonne J P
Laboratoire de Recherches Dermatologiques, Université de Nice-Sophia Antipolis, Nice, France.
Cancer Res. 1993 Mar 1;53(5):1175-81.
The histogenesis of neuroendocrine carcinomas of the skin is still controversial. To determine the degree of neural differentiation of these neoplasias, we studied the expression of intermediate filament proteins in tumoral tissues. Expressions of peripherin, the neurofilament protein NF-L, vimentin, and cytokeratin 8 were analyzed by immunohistochemical methods on 12 human primary tumors and 3 tumor xenografts on nude mice. Peripherin was detected in 10 primary tumors by immunofluorescence. The protein and the corresponding messenger RNA were identified by two-dimensional gel electrophoresis and Northern analysis in extracts of an immunofluorescence-negative tumor. Peripherin, NF-L, and cytokeratin 8 were detected in tumoral cells, whereas vimentin was found exclusively in the stroma. The histological and ultrastructural properties of the original cells of neuroendocrine carcinomas of the skin, as well as coexpression of peripherin, cytokeratin 8, and neurofilament polypeptides, were preserved in tumor xenografts and their primary cultures in vitro. These results bring new elements to the knowledge of the biology of neuroendocrine carcinomas of the skin and indicate that peripherin constitutes a marker for tumor identification.
皮肤神经内分泌癌的组织发生仍存在争议。为了确定这些肿瘤的神经分化程度，我们研究了肿瘤组织中中间丝蛋白的表达。采用免疫组化方法分析了12例人原发性肿瘤和3例裸鼠肿瘤异种移植物中周围蛋白、神经丝蛋白NF-L、波形蛋白和细胞角蛋白8的表达。通过免疫荧光在10例原发性肿瘤中检测到周围蛋白。通过二维凝胶电泳和Northern分析在免疫荧光阴性肿瘤提取物中鉴定了该蛋白及其相应的信使RNA。在肿瘤细胞中检测到周围蛋白、NF-L和细胞角蛋白8，而波形蛋白仅在基质中发现。皮肤神经内分泌癌原始细胞的组织学和超微结构特性，以及周围蛋白、细胞角蛋白8和神经丝多肽的共表达，在肿瘤异种移植物及其体外原代培养物中得以保留。这些结果为皮肤神经内分泌癌的生物学知识带来了新的内容，并表明周围蛋白构成了肿瘤识别的标志物。
