Estival A, Louvel D, Couderc B, Prats H, Hollande E, Vaysse N, Clémente F
INSERM U 151, CHU Rangueil Bat L 3, Toulouse, France.
Cancer Res. 1993 Mar 1;53(5):1182-7.
The role of the different basic fibroblast growth factor (bFGF) forms on the regulation of pancreatic acinar cancer cells was analyzed on the rat cell line AR4-2J. This cell line expresses bFGF receptors but does not produce bFGF. AR4-2J cells were retrovirally transfected with the wild type or with point-mutated bFGF complementary DNAs in order to obtain the expression of all the bFGF forms (clone A4), or of that of the M(r) 22,500 form (clone A3), or of that of the M(r) 18,000 form (clone A5). Each clone was less tumorigenic in nude mice than AR4-2J cells. In culture, only the coexpression of all the bFGF forms modified cell morphology (fibroblast-like) and secretory enzyme synthesis (about a 20-fold decrease of amylase and lipase). Cells expressing the high molecular weight bFGF (A3 and A4) were able to grow in serum-free medium. As for AR4-2J, exogenously added bFGF still exerted mitogenic effects on the bFGF-producing cells. These results suggest that pancreatic acinar cancer cells may respond to endogenous bFGF; furthermore, they seem very sensitive to the coexpression of the different bFGF forms which is often described in cancer cells.
在大鼠细胞系AR4-2J上分析了不同形式的碱性成纤维细胞生长因子（bFGF）对胰腺腺癌细胞调节的作用。该细胞系表达bFGF受体，但不产生bFGF。为了获得所有bFGF形式（克隆A4）、M（r）22,500形式（克隆A3）或M（r）18,000形式（克隆A5）的表达，用野生型或点突变的bFGF互补DNA对AR4-2J细胞进行逆转录病毒转染。每个克隆在裸鼠中的致瘤性均低于AR4-2J细胞。在培养中，只有所有bFGF形式的共表达改变了细胞形态（成纤维细胞样）和分泌酶合成（淀粉酶和脂肪酶减少约20倍）。表达高分子量bFGF（A3和A4）的细胞能够在无血清培养基中生长。与AR4-2J一样，外源添加的bFGF对产生bFGF的细胞仍具有促有丝分裂作用。这些结果表明，胰腺腺癌细胞可能对内源性bFGF有反应；此外，它们似乎对癌细胞中经常描述的不同bFGF形式的共表达非常敏感。
