Lal R B, Buckner C, Khabbaz R F, Kaplan J E, Reyes G, Hadlock K, Lipka J, Foung S K, Chan L, Coligan J E
Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333.
Clin Immunol Immunopathol. 1993 Apr;67(1):40-9. doi: 10.1006/clin.1993.1043.
We have investigated the isotypic and IgG subclass profile of the antibody response to HTLV-I structural proteins (gag and env) in patients with HTLV-I-associated myelopathy (HAM; n = 20), adult T-cell leukemia (ATL; n = 15), and HTLV-I-positive asymptomatic carriers (ASY; n = 21). IgG, IgM, and IgA were the predominant antibody responses in all HTLV-I-infected individuals; minimal IgE response was detectable in the HAM and ATL groups. Among the IgG subclasses, IgG1 was the most predominant antibody detected in responses to HTLV-I antigens, followed by IgG3 and IgG2; IgG4 could not be detected in any patient group. Levels of both IgG1 and IgG3 were significantly higher in patients with HAM, when compared to ATL and ASY (P < 0.01 for both comparisons). In addition, Ig isotypes and IgG subclass antibody in patient sera reactive with purified viral proteins and several immunodominant epitopes, represented by synthetic peptides, Gag-1a102-117, Env-1(191-214), Env-5(242-257), and recombinant proteins, MTA-1(162-209) and r21e303-440, were examined to delineate specific epitopes responsible for inducing the host immune responses of each isotype and subclass to the structural proteins of HTLV-I. IgG, IgM, and IgA responses were directed against both the gag and env gene products. Among IgG subclasses, the IgG1 and IgG3 responses were directed against both the gag (p53, p24, p19, and Gag-1a) and env (recombinant MTA-1, r21e, and synthetic Env-1, Env-5) proteins; IgG2 responses were mainly restricted to gag proteins. The frequency profile of HTLV-I-specific antigen recognition in all four IgG subclasses were similar in all of the clinical groups. These results further define the fine specificity of anti-HTLV-I immune reaction for understanding the mechanism of pathogenesis in these individuals and suggest that factors other than the humoral immune responses may be associated with the clinical manifestation of the disease.
我们研究了人类嗜T淋巴细胞病毒I型（HTLV-I）相关脊髓病（HAM；n = 20）、成人T细胞白血病（ATL；n = 15）和HTLV-I阳性无症状携带者（ASY；n = 21）患者针对HTLV-I结构蛋白（gag和env）的抗体反应的同型和IgG亚类谱。IgG、IgM和IgA是所有HTLV-I感染个体中的主要抗体反应；在HAM和ATL组中可检测到最小的IgE反应。在IgG亚类中，IgG1是对HTLV-I抗原反应中检测到的最主要抗体，其次是IgG3和IgG2；在任何患者组中均未检测到IgG4。与ATL和ASY相比，HAM患者的IgG1和IgG3水平均显著更高（两次比较P均<0.01）。此外，检测了患者血清中与纯化病毒蛋白以及以合成肽Gag-1a102-117、Env-1(191-214)、Env-5(242-257)和重组蛋白MTA-1(162-209)和r21e303-440为代表的几个免疫显性表位反应的Ig同型和IgG亚类抗体，以确定负责诱导宿主对HTLV-I结构蛋白的每种同型和亚类免疫反应的特异性表位。IgG、IgM和IgA反应均针对gag和env基因产物。在IgG亚类中，IgG1和IgG3反应针对gag（p53、p24、p19和Gag-1a）和env（重组MTA-1、r21e以及合成的Env-1、Env-5）蛋白；IgG2反应主要限于gag蛋白。所有四个IgG亚类中HTLV-I特异性抗原识别的频率谱在所有临床组中相似。这些结果进一步明确了抗HTLV-I免疫反应的精细特异性，以了解这些个体的发病机制，并表明除体液免疫反应外的因素可能与疾病的临床表现有关。
