Sakamoto N, Sato C, Haritani H, Maekawa S, Kurosaki M, Enomoto N, Hoshino Y, Tazawa J, Nishimura M, Marumo F
Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan.
J Hepatol. 1993 Jan;17(1):28-33. doi: 10.1016/s0168-8278(05)80517-3.
To determine the prevalence of hepatitis C viral infection in patients with sporadic non-A, non-B (NANB) acute hepatitis, hepatitis C viral RNA was studied in the plasma of 15 patients by reverse transcription-polymerase chain reaction assay. Plasma samples were sequentially obtained from 15 patients, and polymerase chain reaction was performed with two nested pairs of primers deduced from the 5'-non-coding region of hepatitis C viral sequences. Anti-C100 and anti-GOR antibodies were also measured with an enzyme-linked immunosorbent assay system. Plasma hepatitis C viral RNA was detected transiently in 7 of 15 patients (47%) at an early phase of the clinical course, while anti-C100 antibodies were detectable in only 2 (29%) of hepatitis C viral RNA-positive patients, and in 1 (13%) of the negative patients. Of 7 patients that were positive for plasma hepatitis C viral RNA, 4 (57%) had relapsing or protracted courses. In contrast, in all patients with undetectable hepatitis C viral RNA, hepatitis C viral RNA recovered and remained normal for at least 1 year. Thus, hepatitis C viral infection represents almost half the patients with acute sporadic NANB hepatitis, and detection of hepatitis C viral RNA in an early clinical phase is superior to anti-C100 measurement for diagnosing acute sporadic hepatitis C viral infection.
为确定散发性非甲非乙型（NANB）急性肝炎患者中丙型肝炎病毒感染的患病率，采用逆转录 - 聚合酶链反应法对15例患者血浆中的丙型肝炎病毒RNA进行了研究。从15例患者中依次采集血浆样本，并用从丙型肝炎病毒序列5' - 非编码区推导的两对巢式引物进行聚合酶链反应。还使用酶联免疫吸附测定系统检测抗C100和抗GOR抗体。在临床病程早期，15例患者中有7例（47%）血浆丙型肝炎病毒RNA呈短暂性检测阳性，而在丙型肝炎病毒RNA阳性患者中仅有2例（29%）可检测到抗C100抗体，在阴性患者中有1例（13%）可检测到。在血浆丙型肝炎病毒RNA阳性的7例患者中，4例（57%）有复发或迁延病程。相比之下，在所有丙型肝炎病毒RNA检测不到的患者中，丙型肝炎病毒RNA恢复且至少1年内保持正常。因此，丙型肝炎病毒感染几乎占散发性急性NANB肝炎患者的一半，在临床早期检测丙型肝炎病毒RNA对于诊断急性散发性丙型肝炎病毒感染优于检测抗C100。
