使用竞争性聚合酶链反应对肝脏和血清样本中的丙型肝炎病毒RNA进行定量分析。
Quantitation of hepatitis C viral RNA in liver and serum samples using competitive polymerase chain reaction.
作者信息
Sugano M, Hayashi Y, Yoon S, Kinoshita M, Ninomiya T, Ohta K, Itoh H, Kasuga M
机构信息
2nd Department of Internal Medicine, Faculty of Medicine, Kobe University, Japan.
出版信息
J Clin Pathol. 1995 Sep;48(9):820-5. doi: 10.1136/jcp.48.9.820.
AIMS
To investigate whether the amount of hepatitis C viral RNA (HCV-RNA) in liver and serum can predict the effectiveness of interferon treatment in patients with chronic hepatitis C.
METHODS
The amount of HCV-RNA in frozen liver tissues and sera of 22 patients with chronic hepatitis C was determined before and after interferon alfa treatment by the competitive reverse transcriptional polymerase chain reaction method.
RESULTS
Patients with small amounts of HCV-RNA in serum before treatment showed a significantly more effective response to interferon treatment. After treatment, HCV-RNA disappeared from both the liver and serum of all patients who sustained complete response (n = 11); in contrast, HCV-RNA decreased but persisted in the liver of all those who relapsed after cessation of treatment (n = 11).
CONCLUSIONS
The elimination of hepatitis C virus from the liver as well as from the serum seems to be essential for sustained remission. The quantitation of HCV-RNA in liver biopsy specimens obtained after treatment would be a highly accurate predictor of whether relapse is likely to occur.
目的
研究肝组织和血清中丙型肝炎病毒核糖核酸（HCV-RNA）的含量是否能够预测慢性丙型肝炎患者干扰素治疗的效果。
方法
采用竞争性逆转录聚合酶链反应法，测定22例慢性丙型肝炎患者在接受α干扰素治疗前后其冷冻肝组织和血清中的HCV-RNA含量。
结果
治疗前血清中HCV-RNA含量少的患者对干扰素治疗的反应明显更有效。治疗后，所有获得完全缓解的患者（n = 11）肝脏和血清中的HCV-RNA均消失；相反，所有治疗停止后复发的患者（n = 11）肝脏中的HCV-RNA减少但仍持续存在。
结论
从肝脏和血清中清除丙型肝炎病毒似乎是持续缓解的关键。治疗后肝活检标本中HCV-RNA的定量检测将是预测复发可能性的高度准确指标。
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