Le Douarin N M
Institut d'Embryologie cellulaire et moléculaire, CNRS, Collège de France, Nogent-sur-Marne.
Trends Neurosci. 1993 Feb;16(2):64-72. doi: 10.1016/0166-2236(93)90019-i.
The quail-chick cell-marking technique consists of constructing chimaeras in ovo by grafting quail embryonic rudiments into chick embryos or vice versa. Quail and chick cells can be recognized at any time in the chimeras owing to the structures of their interphase nuclei that can be visualized after cytological staining of the DNA. Recently, antibodies that recognize species-specific determinants carried by either neuronal cell bodies or neurites of one or other of the species have greatly enhanced the analytic capacities of this technique, particularly for studying brain development. This article describes the application of the quail-chick chimaera technique to the study of the development of the cerebellum and the optic tectum in the avian embryo. The use of the interspecific chimaeras for behavioural studies is also illustrated by experiments in which certain genetic characteristics of the quail song pattern have been transferred to the chick by neural transplants.
鹌鹑 - 小鸡细胞标记技术包括通过将鹌鹑胚胎原基移植到小鸡胚胎中或反之，在卵内构建嵌合体。由于其间期核的结构，在对DNA进行细胞学染色后可以观察到，因此在嵌合体中的任何时候都可以识别鹌鹑和小鸡细胞。最近，识别由一种或另一种物种的神经元细胞体或神经突携带的物种特异性决定簇的抗体极大地提高了该技术的分析能力，特别是在研究脑发育方面。本文描述了鹌鹑 - 小鸡嵌合体技术在鸟类胚胎小脑和视顶盖发育研究中的应用。通过神经移植将鹌鹑鸣叫模式的某些遗传特征转移到小鸡身上的实验，也说明了种间嵌合体在行为研究中的应用。
