Zanetti M, Lenert G, Springer G F
Department of Medicine, University of California, San Diego 92103.
Int Immunol. 1993 Feb;5(2):113-9. doi: 10.1093/intimm/5.2.113.
All humans normally possess antibodies, predominantly IgM, that react specifically with the Thomsen-Friedenreich (T) and the Tn antigens which are present in immunoreactive form on > 85% of all human carcinomas, but not in healthy and otherwise diseased tissues. We report here a serological study of idiotype expression and antigen reactivity of the anti-T and anti-Tn antibodies. Idiotypy was analyzed with rabbit antibodies raised against, and made specific for, affinity-purified polyclonal anti-T and anti-Tn antibodies from blood group A1B healthy adult donors. Anti-T and anti-Tn antibodies cross-reacted idiotypically in spite of their distinct epitope specificities. By adsorbing anti-T antibodies on insolubilized synthetic T carbohydrate we could firmly link idiotype expression with antigen reactivity. The relation of idiotype expression to the antigen-binding site of plant seed lectins was also studied; one originated from Arachis hypogaea [peanut agglutinin (PNA)], the other from Artocarpus integrifolia (Jacalin). PNA inhibited only anti-T antibodies. Jacalin inhibited both anti-T and anti-Tn antibodies in a dose-dependent manner. Neither idiotypic nor anti-idiotypic antibodies diminished the binding of lectins to T and Tn epitopes. The shared idiotypes on natural anti-T and anti-Tn antibodies permit consideration of application of their anti-idiotypes in treatment and/or prevention of human carcinoma.
所有人类通常都拥有抗体，主要是IgM，这些抗体能与汤姆森 - 弗里德赖希（T）抗原和Tn抗原发生特异性反应，这些抗原以免疫反应形式存在于超过85%的人类癌症中，但在健康组织和其他患病组织中不存在。我们在此报告一项关于抗T和抗Tn抗体的独特型表达及抗原反应性的血清学研究。使用针对A1B血型健康成年供体的亲和纯化多克隆抗T和抗Tn抗体产生并使之特异的兔抗体来分析独特型。尽管抗T和抗Tn抗体具有不同的表位特异性，但它们在独特型上发生交叉反应。通过将抗T抗体吸附在不溶性合成T碳水化合物上，我们能够将独特型表达与抗原反应性紧密联系起来。还研究了独特型表达与植物种子凝集素抗原结合位点的关系；一种凝集素源自落花生（花生凝集素，PNA），另一种源自面包树（杰克豆凝集素）。PNA仅抑制抗T抗体。杰克豆凝集素以剂量依赖方式抑制抗T和抗Tn抗体。独特型抗体和抗独特型抗体均未减少凝集素与T和Tn表位的结合。天然抗T和抗Tn抗体上共享的独特型使得考虑将其抗独特型抗体应用于人类癌症的治疗和/或预防成为可能。
