Duong T, Doucette T, Zidenberg N A, Jacobs R W, Scheibel A B
Indiana University School of Medicine, Terre Haute Center for Medical Education, Terre Haute 47809.
Brain Res. 1993 Feb 12;603(1):74-86. doi: 10.1016/0006-8993(93)91301-8.
The localization of the intracerebral microtubule-associated proteins tau (MAP-tau) has been compared to that of amyloid P component (AP), an extracerebral protein, by single- and double-antigen immunohistochemistry in neurofibrillary tangles of Alzheimer's brains. The results show that, individually, MAP-tau and AP may be observed in all stages of neurofibrillary tangle (NFT) formation. However, NFT labeled by MAP-tau and those labeled by AP largely do not overlap in their distribution. Furthermore, within the few NFT double-labeled by MAP-tau and AP, there was an inverse relationship between the immunoreactivity to MAP-tau and to AP. It is suggested that MAP-tau and AP are incorporated at different times into NFT and that this difference in the timing of NFT expression of these 2 proteins may be useful in the study of progressive NFT formation.
通过单抗原和双抗原免疫组织化学方法，在阿尔茨海默病患者大脑的神经原纤维缠结中，对脑内微管相关蛋白tau（MAP-tau）和一种脑外蛋白——淀粉样P成分（AP）的定位进行了比较。结果显示，单独来看，MAP-tau和AP可在神经原纤维缠结（NFT）形成的各个阶段观察到。然而，由MAP-tau标记的NFT和由AP标记的NFT在分布上基本不重叠。此外，在少数由MAP-tau和AP双标记的NFT中，对MAP-tau和对AP的免疫反应性呈负相关。这表明MAP-tau和AP在不同时间被纳入NFT，这两种蛋白在NFT表达时间上的差异可能有助于研究进行性NFT的形成。
