Ansai S, Hashimoto H, Aoki T, Hozumi Y, Aso K
Department of Dermatology, Yamagata University, Faculty of Medicine, Japan.
Histopathology. 1993 Feb;22(2):127-33. doi: 10.1111/j.1365-2559.1993.tb00090.x.
A histochemical and immunohistochemical study of five cases of extra-ocular sebaceous carcinoma was performed using formalin-fixed and paraffin-embedded tissue specimens. Histochemically, the clear cells of sebaceous carcinomas were negative with periodic acid-Schiff and alcian blue staining. Immunohistochemically, the tumour cells of sebaceous carcinomas showed positive reactions for epithelial membrane antigen, human milk fat globules subclass 1, human milk fat globules subclass 2 and Leu M1, but did not express carcinoembryonic antigen, breast carcinoma associated antigen, S-100 protein, gross cystic disease fluid protein-15 or Dako M1. These histochemical and immunohistochemical findings were compared with those of other skin cancers which must be distinguished histopathologically from sebaceous carcinoma. We conclude that sebaceous carcinoma can be distinguished from eccrine porocarcinoma, malignant clear cell hidradenoma, extramammary Paget's disease, malignant trichilemmoma, squamous cell carcinoma and basal cell carcinoma by histochemical and immunohistochemical techniques using formalin-fixed and paraffin-embedded tissue specimens.
使用福尔马林固定和石蜡包埋的组织标本，对5例眼外皮脂腺癌进行了组织化学和免疫组织化学研究。组织化学方面，皮脂腺癌的透明细胞对过碘酸希夫染色和阿尔辛蓝染色呈阴性。免疫组织化学方面，皮脂腺癌的肿瘤细胞对上皮膜抗原、人乳脂肪球亚类1、人乳脂肪球亚类2和Leu M1呈阳性反应，但不表达癌胚抗原、乳腺癌相关抗原、S-100蛋白、巨大囊肿病液体蛋白-15或Dako M1。将这些组织化学和免疫组织化学结果与其他必须通过组织病理学与皮脂腺癌相鉴别的皮肤癌的结果进行了比较。我们得出结论，使用福尔马林固定和石蜡包埋的组织标本，通过组织化学和免疫组织化学技术，可以将皮脂腺癌与小汗腺汗孔癌、恶性透明细胞汗腺瘤、乳腺外佩吉特病、恶性毛鞘瘤、鳞状细胞癌和基底细胞癌区分开来。
