Heyderman E, Graham R M, Chapman D V, Richardson T C, McKee P H
Histopathology. 1984 May;8(3):423-34. doi: 10.1111/j.1365-2559.1984.tb02354.x.
Sixty-five primary malignant skin tumours have been stained for carcinoembryonic antigen (CEA) and epithelial membrane antigen (EMA) using rabbit polyclonal affinity-purified antibodies and an indirect immunoperoxidase technique. The tumours consisted of 15 invasive squamous carcinomas, 23 basal cell carcinomas, 16 malignant eccrine poromas (porocarcinomas), and 11 sebaceous carcinomas. The basal cell carcinomas were negative for CEA and EMA except where there was keratotic or sebaceous differentiation. All the sebaceous and squamous carcinomas and 15/16 porocarcinomas contained EMA. 12/15 squamous carcinomas were positive for CEA. The malignant poromas were negative for CEA except on the ulcerated surface of two. In tumours classified as sebaceous carcinomas there was positive staining for CEA in some cells, cyst contents and/or keratotic foci. These findings have implications for the use of immunoperoxidase localization of epithelial markers in the differential diagnosis of primary and metastatic skin cancer.
使用兔多克隆亲和纯化抗体和间接免疫过氧化物酶技术，对65例原发性恶性皮肤肿瘤进行癌胚抗原（CEA）和上皮膜抗原（EMA）染色。这些肿瘤包括15例浸润性鳞状细胞癌、23例基底细胞癌、16例恶性小汗腺汗孔瘤（汗孔癌）和11例皮脂腺癌。基底细胞癌除有角化或皮脂腺分化处外，CEA和EMA均为阴性。所有皮脂腺癌和鳞状细胞癌以及16例汗孔癌中的15例含有EMA。15例鳞状细胞癌中有12例CEA呈阳性。恶性汗孔瘤除2例的溃疡表面外，CEA均为阴性。在分类为皮脂腺癌的肿瘤中，一些细胞、囊肿内容物和/或角化灶CEA染色呈阳性。这些发现对免疫过氧化物酶定位上皮标志物在原发性和转移性皮肤癌鉴别诊断中的应用具有重要意义。
