Ansai S, Katagata Y, Yoshikawa K, Hashimoto H, Hozumi Y, Kondo S, Aso K
Department of Dermatology, Yamagata University, Faculty of Medicine, Japan.
J Dermatol. 1994 Aug;21(8):553-9. doi: 10.1111/j.1346-8138.1994.tb01793.x.
Formalin-fixed and paraffin-embedded tissue specimens of six cases of extraocular sebaceous carcinoma were studied immunohistochemically with eight anti-keratin monoclonal antibodies, 34 beta B4, 35 beta H11, Ks13.1, Ks19.1, PKK1, LP34, KL1 and AE1. The staining patterns of sebaceous carcinoma were compared with those of normal sebaceous glands and other skin cancers which should be distinguished from sebaceous carcinoma histopathologically. The other skin cancers compared were eccrine porocarcinoma, malignant clear cell hidradenoma, extramammary Paget's disease with underlying adenocarcinoma, malignant trichilemmoma, and squamous cell carcinoma. Most cases of sebaceous carcinoma were stained with 35 beta H11, Ks19.1, LP34, KL1 and AE1, while normal sebaceous glands were positive only with 35 beta H11, LP34, KL1 and AE1. By immunostaining, sebaceous carcinoma was distinguishable from extramammary Paget's disease with underlying adenocarcinoma, squamous cell carcinoma, malignant trichilemmoma, and eccrine porocarcinoma, but was not clearly distinguishable from malignant clear cell hidradenoma. These findings demonstrate that sebaceous carcinoma shows positive reactions with antibodies to simple epithelial keratin, probably as a result of neoplastic transformation, and that immunohistochemical examination using anti-keratin monoclonal antibodies is useful in distinguishing sebaceous carcinoma from several other skin cancers.
对6例眼外皮脂腺癌的福尔马林固定石蜡包埋组织标本，用8种抗角蛋白单克隆抗体34βB4、35βH11、Ks13.1、Ks19.1、PKK1、LP34、KL1和AE1进行免疫组织化学研究。将皮脂腺癌的染色模式与正常皮脂腺以及其他在组织病理学上应与皮脂腺癌相鉴别的皮肤癌的染色模式进行比较。所比较的其他皮肤癌包括小汗腺汗孔癌、恶性透明细胞汗腺瘤、伴潜在腺癌的乳腺外佩吉特病、恶性毛鞘瘤和鳞状细胞癌。大多数皮脂腺癌病例用35βH11、Ks19.1、LP34、KL1和AE1染色，而正常皮脂腺仅对35βH11、LP34、KL1和AE1呈阳性。通过免疫染色，皮脂腺癌可与伴潜在腺癌的乳腺外佩吉特病、鳞状细胞癌、恶性毛鞘瘤和小汗腺汗孔癌相鉴别，但与恶性透明细胞汗腺瘤无明显区别。这些发现表明，皮脂腺癌对简单上皮角蛋白抗体呈阳性反应，这可能是肿瘤转化的结果，并且使用抗角蛋白单克隆抗体的免疫组织化学检查有助于将皮脂腺癌与其他几种皮肤癌区分开来。
