Martín Sánchez A M, Rodríguez Hernández J, Canut Blasco A, Dovigo Prieto C
Departamento de Ciencias Clínicas, Facultad de Ciencias Médicas, Universidad de las Palmas de Gran Canaria.
Rev Clin Esp. 1993 Feb;192(2):63-6.
The usefulness of five dying techniques to detect oocysts of Cryptosporidium spp in stools is evaluated in a healthy children preschool population, taking from a smear of the sediment obtained in the formalin-ether concentration technique (Ritchie's test). 10 positive stool samples were detected of a total of 115 samples (8.7%). Dying with Lugol the sediment of Ritchie's test only detected 40% of positive samples. Modified Ziehl-Neelsen dye, auramine dye and indirect immunofluorescence dye with monoclonal antibodies, yielded an excellent diagnostic correlation (each of them detected 90% of the positive samples). Indirect immunofluorescence dye with specific human antibodies detected one case more of parasites than the rest of dyes, is not expensive and easy to perform.
在一个健康儿童学龄前人群中，从用福尔马林-乙醚浓缩技术（里奇氏试验）获得的沉淀物涂片上，评估了五种染色技术检测粪便中隐孢子虫属卵囊的有效性。在总共115份样本中检测到10份阳性粪便样本（8.7%）。用卢戈氏碘液对里奇氏试验的沉淀物进行染色，仅检测到40%的阳性样本。改良齐尔-尼尔森染色、金胺染色和单克隆抗体间接免疫荧光染色产生了极佳的诊断相关性（每种方法都检测到90%的阳性样本）。用特异性人抗体进行的间接免疫荧光染色比其他染色方法多检测到一例寄生虫，且成本不高且易于操作。
