Chichino G, Bruno A, Cevini C, Atzori C, Gatti S, Scaglia M
Department of Infectious Diseases, University, IRCCS San Matteo, Pavia, Italy.
J Protozool. 1991 Nov-Dec;38(6):212S-214S.
Two new extemporaneous negative-staining methods are proposed to detect Cryptosporidium oocysts in stools, using light-green and merbromine in 1% and 2% aqueous solution, respectively. A Ziehl-Neelsen stain as modified by Henriksen and Polhenz was used as a reference technique. A comparison between these two new stains and the reference, a modified Ziehl-Neelsen mod. method gave almost identical sensitivity and specificity. We propose their use in routine diagnosis for enteric cryptosporidiosis.
