Okita K, Li Q, Murakamio T, Takahashi M
First Department of Internal Medicine, Yamaguchi University School of Medicine, Japan.
Eur J Cancer Prev. 1993 Mar;2(2):169-75. doi: 10.1097/00008469-199303000-00010.
The present study was undertaken to investigate the effects and the mechanism of the components of Sho-saiko-to (baicalein, baicalin, saikosaponin-a, saikosaponin-c, ginsenoside Rb1, ginsenoside Rg1) on cultured human hepatoma cells (HuH-7). Cell cycle analysis was carried out with flow cytometry and the bromodeoxyuridine (BrdU)-labelling method. The results showed that baicalein, baicalin and saikosaponin-a inhibited cell proliferation dose-dependently but independently of the cell cycle. Furthermore, it was found that saikosaponin-a possesses a strong cell-killing effect. On the other hand, saikosaponin-c, ginsenoside Rb1 and ginsenoside Rg1 had no effect on cell proliferation.
本研究旨在探讨小柴胡汤的成分（黄芩素、黄芩苷、柴胡皂苷a、柴胡皂苷c、人参皂苷Rb1、人参皂苷Rg1）对培养的人肝癌细胞（HuH-7）的影响及其作用机制。采用流式细胞术和溴脱氧尿苷（BrdU）标记法进行细胞周期分析。结果表明，黄芩素、黄芩苷和柴胡皂苷a剂量依赖性地抑制细胞增殖，但与细胞周期无关。此外，还发现柴胡皂苷a具有很强的细胞杀伤作用。另一方面，柴胡皂苷c、人参皂苷Rb1和人参皂苷Rg1对细胞增殖没有影响。
