Dörries R, ter Meulen V
J Gen Virol. 1983 Jan;64 (Pt 1):159-67. doi: 10.1099/0022-1317-64-1-159.
The specificity of the IgM response in acute human coxsackievirus B infections was examined by indirect solid phase enzyme immunoassay and immunoblot techniques. IgM antibodies detected by ELISA were either strictly type-specific, type-predominant or group-reactive to coxsackieviruses B-1 to B-5. Homotypic and type-dominant responses were clearly correlated with the serotype isolated from the patient. Analysis of ELISA antigens by SDS-gel electrophoresis, protein transfer and subsequent immunodetection of individual virus polypeptides, revealed VP1 as the major antigen; it was detected by homotypic as well as heterotypic serum specimens.
采用间接固相酶免疫测定法和免疫印迹技术检测了急性人类柯萨奇病毒B感染中IgM反应的特异性。通过酶联免疫吸附测定法检测到的IgM抗体对柯萨奇病毒B-1至B-5要么具有严格的型特异性、型优势性，要么具有群反应性。同型和型优势反应与从患者分离出的血清型明显相关。通过十二烷基硫酸钠 - 凝胶电泳、蛋白质转移以及随后对单个病毒多肽的免疫检测对酶联免疫吸附测定抗原进行分析，结果显示VP1是主要抗原；同型和异型血清标本均可检测到该抗原。
