Snoeck H W, Lardon F, van Bockstaele D R, Peetermans M E
Laboratory of Experimental Hematology, University of Antwerp (UZA), Belgium.
Leukemia. 1993 Apr;7(4):625-9.
We studied the effects of interleukin 4 (IL4) on myelopoiesis supported by either granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage CSF (GM-CSF) or IL3 on purified CD34+ bone marrow progenitor cells. IL4 stimulates the colony-forming unit granulocyte (CFU-G) induced by G-CSF and inhibits all colony types supported by either IL3 and GM-CSF, although inhibition of CFU-M (macrophage) was significantly stronger than that of CFU-G and CFU-GM. When the cells were first incubated in liquid culture for 4 days in IL4, followed by agar culture in G-CSF, there was a significant increase in the number of CFU-G compared to cells which had been incubated in medium alone for 4 days before plating out in agar containing G-CSF. The inhibitory effects of IL4 on GM-CSF or IL3 supported colony formation, however, disappear with sequential incubation in IL4 in liquid culture followed by culture in agar with either GM-CSF or IL3.
我们研究了白细胞介素4（IL4）对由粒细胞集落刺激因子（G-CSF）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）或IL3支持的纯化CD34+骨髓祖细胞髓系造血的影响。IL4刺激由G-CSF诱导的粒细胞集落形成单位（CFU-G），并抑制由IL3和GM-CSF支持的所有集落类型，尽管对CFU-M（巨噬细胞）的抑制明显强于对CFU-G和CFU-GM的抑制。当细胞先在IL4中进行4天的液体培养，然后在G-CSF中进行琼脂培养时，与在含G-CSF的琼脂中接种前仅在培养基中培养4天的细胞相比，CFU-G的数量显著增加。然而，IL4对GM-CSF或IL3支持的集落形成的抑制作用，在液体培养中依次在IL4中培养，然后在含GM-CSF或IL3的琼脂中培养后消失。
