Cultured mouse SR-1 cells exposed to low dose of gamma-rays become less susceptible to the induction of mutagenesis by radiation as well as bleomycin.

The effect of pre-exposure of cultured mouse SR-1 cells to a low dose of gamma-rays on the induction of mutations at the hprt locus by subsequent high dose radiation or bleomycin was studied. When cells were pre-exposed to 0.01 Gy gamma-rays, the induction of mutations by a 3 Gy gamma-ray dose given 18 or 24 h later was significantly reduced as compared with those which did not receive the low dose pre-exposure. When cells were challenged with 5 or 10 micrograms/ml bleomycin for 12 h, which can produce double-strand breaks in DNA, instead of 3 Gy gamma-rays, a similar mutagenetic adaptive response was observed. We conclude that this resistance to radiation- or bleomycin-induced mutation is attributed to the induction of a DNA double-strand break repair mechanism.