Morris J L, Gibbins I L
Department of Anatomy and Histology, Flinders University of South Australia, Bedford Park.
J Neurosci. 1987 Oct;7(10):3117-30. doi: 10.1523/JNEUROSCI.07-10-03117.1987.
The patterns of colocalization of neuropeptides, catecholamines, and catecholamine-synthesizing enzymes were examined in principal neurons and nerve terminals in guinea pig paracervical ganglia using a double-labeling immunohistochemical procedure. A small proportion of nerve cell bodies (less than 10%) had the characteristics of catecholamine-synthesizing neurons and presumably were noradrenergic. Another 50% of the nerve cell bodies contained immunoreactivity (IR) to dopamine-beta-hydroxylase (DBH), but did not have any other characteristics of noradrenergic neurons; they did not contain detectable catecholamines, or IR to dopa decarboxylase (DDC) or tyrosine (TH) hydroxylase, nor did they take up exogenous catecholamines. Half of the catecholamine neurons had neuropeptide Y (NPY)-IR, and a small number (0.5% total neurons) had somatostatin (Som)-IR. Most of the non-noradrenergic neurons with DBH-IR (40-50% total neurons) contained IR for dynorphin (Dyn), NPY, and vasoactive intestinal peptide (VIP), and about half of them (20-25% total) also contained Som-IR. Ten to twenty percent of neurons contained IR to Som, but not to any other antigen examined here. Nerve terminals with substance P (SP)-IR or enkephalin (Enk)-IR were prominent in all ganglia. SP-IR fibers formed dense baskets only around those neurons with DBH/Dyn/NPY/VIP (+/- Som)-IR, while fibers with very bright Enk-IR were associated selectively with those neurons with Som-IR alone. In addition, most TH-IR nerve cell bodies were surrounded by NPY-IR varicose nerve fibers. In conclusion, this analysis of combinations of peptides and enzymes contained in principal neurons of the paracervical ganglia allows us to identify as many as 11 different neuron populations. The functional significance of the presence of the same neuropeptide (e.g., NPY) in different neuron populations is as yet unknown. Some of these classes of neurons are associated specifically with immunohistochemically distinct types of presynaptic nerve fibers, which suggests that different immunohistochemically defined classes of neurons represent different functional pathways.
采用双标记免疫组织化学方法，在豚鼠子宫旁神经节的主要神经元和神经终末中，研究了神经肽、儿茶酚胺和儿茶酚胺合成酶的共定位模式。一小部分神经细胞体（少于10%）具有儿茶酚胺合成神经元的特征，推测为去甲肾上腺素能神经元。另外50%的神经细胞体对多巴胺-β-羟化酶（DBH）有免疫反应性（IR），但不具有去甲肾上腺素能神经元的任何其他特征；它们不含可检测到的儿茶酚胺，对多巴脱羧酶（DDC）或酪氨酸（TH）羟化酶也无IR，也不摄取外源性儿茶酚胺。一半的儿茶酚胺能神经元有神经肽Y（NPY）-IR，少数（占神经元总数的0.5%）有生长抑素（Som）-IR。大多数具有DBH-IR的非去甲肾上腺素能神经元（占神经元总数的40 - 50%）含有强啡肽（Dyn）、NPY和血管活性肠肽（VIP）的IR，其中约一半（占总数的20 - 25%）也含有Som-IR。10%至20%的神经元含有Som-IR，但对这里检测的任何其他抗原均无反应。含有P物质（SP）-IR或脑啡肽（Enk）-IR的神经终末在所有神经节中都很突出。SP-IR纤维仅在那些具有DBH/Dyn/NPY/VIP（±Som）-IR的神经元周围形成密集的篮状结构，而具有非常明亮Enk-IR的纤维则仅与那些单独具有Som-IR的神经元选择性相关。此外，大多数TH-IR神经细胞体被NPY-IR曲张神经纤维包围。总之，对子宫旁神经节主要神经元中所含肽和酶组合的这种分析使我们能够识别多达11种不同的神经元群体。同一神经肽（如NPY）在不同神经元群体中存在的功能意义尚不清楚。这些神经元类别中的一些与免疫组织化学上不同类型的突触前神经纤维特异性相关，这表明不同免疫组织化学定义的神经元类别代表不同的功能途径。
