Villalobos-Arámbula A R, Aguilar-Luna J C, Esparza A, Perea F J, de Loza R, Hernández-Córdova A, Ibarra B
División de Genética, CMNO, IMSS, Guadalajara, Jal., México.
Sangre (Barc). 1993 Feb;38(1):31-5.
To search in children with acute lymphoblastic leukaemia (ALL) for specific pattern of expression of foetal haemoglobin (HbF) and its G gamma/A gamma chain ratio.
60 children with ALL were examined: 29 with ALL-L1, and 31 with ALL-L2, and 25 healthy children as control group, which were subdivided in three groups: A) 0-5, B) 6-10 and C) 11-18 years. We performed HbF and HbA2 quantification and Hb electrophoresis. G gamma and A gamma globin chain percentages were obtained with a new method based on the precipitation of the HbF eluate by Singer's method with sulphosalycilic acid, the globin chains were separated in polyacrylamide with Triton X-100 and quantified by densitometry.
HbF showed similar levels in both ALL groups by the Betke and Singer's methods; (ALL-L1: 2.2 +/- 1.5%, ALL-L2: 2.0 +/- 1.2%; and ALL-L1: 2.0 +/- 1.2%, ALL-L2: 2.1 +/- 1.5% respectively), but there were statistically significant differences (p < 0.001) when compared with the control group (0.9 +/- 0.4%, and 1.0 +/- 0.6% for Betke and Singer's method). The G gamma/A gamma ratio showed to be different between the ALL-L1 and ALL-L2 (p < 0.001), with higher levels of G gamma in ALL-L1 (51.0%), the ALL-L2 and the control group showed similar G gamma values (37.5% and 42.1% respectively).
The factors involved in the increase of HbF are similar for both ALL-L1 and ALL-L2. However there seems to be different factors affecting the expression of G gamma or A gamma.
在急性淋巴细胞白血病（ALL）患儿中寻找胎儿血红蛋白（HbF）的特定表达模式及其Gγ/Aγ链比值。
对60例ALL患儿进行检查：29例ALL-L1型，31例ALL-L2型，25例健康儿童作为对照组，对照组又分为三组：A）0 - 5岁，B）6 - 10岁，C）11 - 18岁。我们进行了HbF和HbA2定量以及血红蛋白电泳。采用一种基于辛格法用磺基水杨酸沉淀HbF洗脱液的新方法获得Gγ和Aγ珠蛋白链百分比，珠蛋白链在含Triton X - 100的聚丙烯酰胺中分离并通过光密度法进行定量。
通过贝特克法和辛格法，两组ALL患儿的HbF水平相似；（ALL-L1型：2.2±1.5%，ALL-L2型：2.0±1.2%；ALL-L1型：2.0±1.2%，ALL-L2型：2.1±1.5%），但与对照组相比有统计学显著差异（p < 0.001）（贝特克法和辛格法对照组分别为0.9±0.4%和1.0±0.6%）。ALL-L1型和ALL-L2型的Gγ/Aγ比值不同（p < 0.001），ALL-L1型中Gγ水平较高（51.0%），ALL-L2型和对照组的Gγ值相似（分别为37.5%和42.1%）。
ALL-L1型和ALL-L2型中导致HbF升高的因素相似。然而，似乎有不同因素影响Gγ或Aγ的表达。
