乳腺及乳腺外佩吉特病中角蛋白型中间丝的免疫组织化学染色特性
Immunohistochemical staining properties of keratin type intermediate filaments in mammary and extramammary Paget's disease.
作者信息
Yoneda K
出版信息
J Dermatol. 1989 Feb;16(1):47-53. doi: 10.1111/j.1346-8138.1989.tb01218.x.
Utilizing three different anti-keratin antibodies and the avidin-biotin peroxidase complex system on sections of formalin-fixed paraffin-embedded tissues and on cryostat sections, immunohistochemical localization of keratin type intermediate filaments in mammary Paget's disease and extramammary Paget's disease was investigated. Anti-keratin antibodies EAB-903 and EAB-904, which recognize 66K and 57K dalton keratin peptides, did not decorate any Paget's cells in either mammary or extramammary Paget's disease. On the other hand, anti-keratin antibody MAK-6, which recognizes 52.5K, 50K, 48K, 45K and 40K daltons keratin peptides, did decorate Paget's cells in both Paget's diseases. These staining properties of Paget's cells were the same as those of secretory cells in normal human sweat glands and mammary glands. Anti-keratin antibody MAK-6 is thought to be useful in the diagnosis of mammary and extramammary Paget's diseases.
利用三种不同的抗角蛋白抗体以及抗生物素蛋白-生物素过氧化物酶复合物系统，对福尔马林固定石蜡包埋组织切片和低温恒温器切片进行检测，研究了乳腺佩吉特病和乳腺外佩吉特病中角蛋白型中间丝的免疫组织化学定位。识别66K和57K道尔顿角蛋白肽的抗角蛋白抗体EAB - 903和EAB - 904，在乳腺或乳腺外佩吉特病中均未标记任何佩吉特细胞。另一方面，识别52.5K、50K、48K、45K和40K道尔顿角蛋白肽的抗角蛋白抗体MAK - 6，在两种佩吉特病中均标记了佩吉特细胞。佩吉特细胞的这些染色特性与正常人汗腺和乳腺中的分泌细胞相同。抗角蛋白抗体MAK - 6被认为在乳腺和乳腺外佩吉特病的诊断中有用。
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