Kim Y H, Buchholz M J, Nordin A A
Clinical Immunology Section, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224.
Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3187-91. doi: 10.1073/pnas.90.8.3187.
The addition of recombinant interleukin 2 (rIL-2) to anti-CD3-activated murine G0 phase T cells results in an increased level of tyrosine phosphorylation of a single 97-kDa protein. The degree of tyrosine phosphorylation paralleled the amount of rIL-2 added and correlated with the extent of DNA synthesis. IL-2 treatment resulted in a transient increase in p56lck kinase activity without detectable modification of its level of tyrosine phosphorylation and gel mobility. When G0 T cells were activated by phorbol dibutyrate in the absence of IL-2, the high-affinity IL-2 receptor (IL-2R) expressed failed to induce a proliferative signal, and neither the tyrosine phosphorylation of the 97-kDa protein nor the transient increase in p56lck kinase activity was detected. Northern analysis of the total RNA extracted from these cells showed the accumulation of IL-2R alpha chain-specific mRNA but neither c-myc nor cdc2 mRNA was expressed. The addition of 100 nM rIL-2 to T cells activated by phorbol dibutyrate was able to induce a proliferative response, and under these conditions tyrosine phosphorylation of the 97-kDa protein, the transient increase in p56lck kinase activity, and specific mRNA for IL-2R alpha chain, c-myc, and cdc2 were detected. Unstimulated G0 T cells responded to 100 nM rIL-2 in the same manner as phorbol dibutyrate-activated cells. Irrespective of the signal-transducing structures involved, the IL-2-induced proliferative response closely correlates with an increase in p56lck kinase activity along with the tyrosine phosphorylation of a 97-kDa protein.
将重组白细胞介素2（rIL-2）添加到抗CD3激活的小鼠G0期T细胞中，会导致一种单一的97 kDa蛋白的酪氨酸磷酸化水平升高。酪氨酸磷酸化程度与添加的rIL-2量平行，并与DNA合成程度相关。IL-2处理导致p56lck激酶活性短暂增加，但其酪氨酸磷酸化水平和凝胶迁移率未检测到改变。当G0 T细胞在没有IL-2的情况下被佛波酯二丁酸酯激活时，所表达的高亲和力IL-2受体（IL-2R）未能诱导增殖信号，并且未检测到97 kDa蛋白的酪氨酸磷酸化和p56lck激酶活性的短暂增加。对从这些细胞中提取的总RNA进行Northern分析，显示出IL-2Rα链特异性mRNA的积累，但未表达c-myc和cdc2 mRNA。将100 nM rIL-2添加到被佛波酯二丁酸酯激活的T细胞中能够诱导增殖反应，在这些条件下，检测到97 kDa蛋白的酪氨酸磷酸化、p56lck激酶活性的短暂增加以及IL-2Rα链、c-myc和cdc2的特异性mRNA。未刺激的G0 T细胞对100 nM rIL-2的反应与佛波酯二丁酸酯激活的细胞相同。无论涉及何种信号转导结构，IL-2诱导的增殖反应都与p56lck激酶活性的增加以及97 kDa蛋白的酪氨酸磷酸化密切相关。
