大鼠18S核糖体核糖核酸基因的核苷酸序列及18S核糖体核糖核酸二级结构的提议

The nucleotide sequence of a rat 18 S ribosomal ribonucleic acid gene and a proposal for the secondary structure of 18 S ribosomal ribonucleic acid.

作者信息

Chan Y L, Gutell R, Noller H F, Wool I G

出版信息

J Biol Chem. 1984 Jan 10;259(1):224-30.

Abstract

The nucleotide sequence of a rat 18 S rRNA gene was determined. The 18 S rRNA encoded in the gene contains 1874 nucleotides, and the molecular weight estimated from the sequence is 6.09 X 10(5). The sequences of rat and Xenopus laevis 18 S rRNAs are very similar; the only differences of consequence are the insertions between nucleotides 197 and 206 and between 258 and 279 of the rat nucleic acid of sequences rich in guanine and cytosine. A proposal is presented for the secondary structure of rat 18 S rRNA based on a comparison with the sequences of 17 other small ribosomal subunit RNAs. While the primary sequences of rat and eubacterial RNAs are different, the deduced secondary structures are remarkably similar.

摘要

测定了大鼠18S rRNA基因的核苷酸序列。该基因编码的18S rRNA含有1874个核苷酸，根据序列估算的分子量为6.09×10⁵。大鼠和非洲爪蟾18S rRNA的序列非常相似；唯一重要的差异是在富含鸟嘌呤和胞嘧啶的大鼠核酸序列中，核苷酸197与206之间以及258与279之间存在插入。基于与其他17种小核糖体亚基RNA序列的比较，提出了大鼠18S rRNA的二级结构。虽然大鼠和真细菌RNA的一级序列不同，但推导的二级结构非常相似。