Kudriavtsev I S, Zatsepina O V
Tsitologiia. 1993;35(1):30-5.
Mouse chromosome behaviour has been studied in the anaphase, after the cells (culture L) were incubated with fluorochrome Hoechst 33258, provoking the stretching of the chromosome centromeric regions. It was shown that in spite of marked structural changes in the centromere, the cells entered the anaphase and chromosomes preserved their ability to separate towards the spindle poles. At the same time, in the presence of Hoechst 33258, a reliable increase in the anaphase duration could be observed from 4.0 +/- 0.2 min in control to 6.0 +/- 0.5 min after a prolonged incubation of cells with the fluorochrome. Also, the increase in the frequency of chromosomal bridges in anaphase and the appearance of bridges in interphase cells were observed.
在用荧光染料Hoechst 33258孵育细胞（培养物L）后，于后期对小鼠染色体行为进行了研究，这会导致染色体着丝粒区域伸展。结果表明，尽管着丝粒有明显的结构变化，但细胞仍进入后期，且染色体保留了向纺锤体极分离的能力。同时，在存在Hoechst 33258的情况下，可以观察到后期持续时间从对照中的4.0±0.2分钟可靠地增加到在用荧光染料长时间孵育细胞后的6.0±0.5分钟。此外，还观察到后期染色体桥频率的增加以及间期细胞中桥的出现。
