Mack G, Rattner J B
Department of Anatomy, University of Calgary, Alberta, Canada.
Cell Motil Cytoskeleton. 1993;26(3):239-47. doi: 10.1002/cm.970260307.
The behaviour of the centrosome immediately following cell division in tissue culture cells has been investigated. We find that following karyokinesis, but preceding cytokinesis, sister centrosomes relocate from the spindle poles to a position adjacent to the intercellular bridge. This repositioning is accompanied by the appearance of a microtubule bundle that extends from the poleward region of the cell to the centrosome and increases in length as the centrosome approaches the intercellular bridge. Disruption of this bundle with colcemid interrupts centrosome repositioning. In contrast, centrosome repositioning persists in late mitotic cells grown in the presence of cytochalasin D. However, the position of the microtubule-centrosome complex within the cell is randomized suggesting that the path, but not the process, of centrosome repositioning is dependent on an intact actin filament network. This study points out, for the first time, that the complex migration of the centrosome preceding mitosis is paralleled by an equally complex set of events following cell division. We suggest that post-mitotic centrosome repositioning may play a role in ensuring that daughter cells have equal but opposite polarity and may reflect an interrelationship between the establishment of the interphase cytoskeleton and the completion of cytokinesis.
研究了组织培养细胞中细胞分裂后中心体的行为。我们发现，在核分裂之后、胞质分裂之前，姐妹中心体从纺锤体极重新定位到与细胞间桥相邻的位置。这种重新定位伴随着一束微管的出现，该微管从细胞的极向区域延伸至中心体，并随着中心体靠近细胞间桥而变长。用秋水仙酰胺破坏这束微管会中断中心体的重新定位。相反，在细胞松弛素D存在的情况下生长的晚期有丝分裂细胞中，中心体重新定位持续存在。然而，细胞内微管 - 中心体复合体的位置是随机的，这表明中心体重新定位的路径而非过程依赖于完整的肌动蛋白丝网络。这项研究首次指出，有丝分裂前中心体的复杂迁移与细胞分裂后同样复杂的一系列事件相平行。我们认为，有丝分裂后中心体重新定位可能在确保子细胞具有相等但相反的极性方面发挥作用，并且可能反映了间期细胞骨架的建立与胞质分裂完成之间的相互关系。
