Trigo F J, Breeze R G, Evermann J F, Gallina A M
Am J Vet Res. 1984 Aug;45(8):1663-70.
Conventionally reared lambs, seronegative to bovine respiratory syncytial virus (RSV), were inoculated with bovine RSV by an aerosol route. A mild clinical response, characterized by transient fever at postinoculation day (PID) 4, occurred and was accompanied by serous oculonasal secretions in less than 50% of the infected lambs. A decrease in total WBC was observed on PID 6 and 11, with a reduction in the total numbers of lymphocytes. Bovine RSV was recovered from nasal secretions of inoculated lambs from PID 2 to 6. The lambs were euthanatized, and at necropsy, the virus was only isolated from lambs killed between PID 2 and 4. Serum neutralizing antibodies were noticed as early as PID 6 and peaked at PID 13 to 15. Neutralizing antibodies were also detected at low concentration in pulmonary washings. Viral antigen was mainly located in alveolar walls and occasionally in bronchiolar epithelium and alveolar macrophages in lambs killed between PID 2 to 4. Later, specific fluorescence was only present in alveolar walls and was not observed in tissue from lambs killed after PID 22. Budding and free virus-like particles were observed by electron microscopy in type I and type II alveolar epithelial cells. Outstanding macroscopic lesions were not seen. Histologically, mild bronchiolitis and alveolitis were observed.
对牛呼吸道合胞病毒（RSV）血清学阴性的传统饲养羔羊，通过气溶胶途径接种牛RSV。出现了以接种后第4天（PID 4）短暂发热为特征的轻度临床反应，不到50%的感染羔羊伴有浆液性眼鼻分泌物。在PID 6和11观察到白细胞总数减少，淋巴细胞总数减少。从接种羔羊的鼻分泌物中在PID 2至6期间分离出牛RSV。羔羊被安乐死，尸检时，仅从PID 2至4期间处死的羔羊中分离到病毒。血清中和抗体最早在PID 6时出现，并在PID 13至15时达到峰值。在肺灌洗液中也检测到低浓度的中和抗体。病毒抗原主要位于肺泡壁，偶尔位于PID 2至4期间处死的羔羊的细支气管上皮和肺泡巨噬细胞中。后来，仅在肺泡壁中出现特异性荧光，在PID 22之后处死的羔羊组织中未观察到。通过电子显微镜在I型和II型肺泡上皮细胞中观察到出芽和游离的病毒样颗粒。未观察到明显的宏观病变。组织学上，观察到轻度细支气管炎和肺泡炎。
