Fenderson B A, Radin N, Andrews P W
Department of Pathology, Thomas Jefferson Medical College, Philadelphia, Pa.
Eur Urol. 1993;23(1):30-6; discussion 36-7. doi: 10.1159/000474567.
Human and mouse embryonal carcinoma (EC) cells are characterized by their expression of cell surface carbohydrate antigens, present in both glycolipids and glycoproteins. These antigens disappear upon differentiation and are replaced with other antigens. We have used the inhibitor of glucosyl ceramide synthetase, 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), to study the distribution of carbohydrate epitopes between glycolipids and glycoproteins. PDMP inhibited the expression of glycolipid antigens, but not glycoprotein antigens assayed by immunofluorescence and thin layer chromatography. In the case of SSEA1, we observed expression on both glycolipids and glycoproteins. Resistance to PDMP inhibition suggests that glycoproteins carry the immunodominant form of SSEA1 on the cell surface of differentiated human EC cells and undifferentiated murine EC cells.
人和小鼠胚胎癌细胞的特征在于其细胞表面碳水化合物抗原的表达，这些抗原存在于糖脂和糖蛋白中。这些抗原在分化时消失，并被其他抗原取代。我们使用了葡糖神经酰胺合成酶抑制剂1-苯基-2-癸酰氨基-3-吗啉代-1-丙醇（PDMP）来研究碳水化合物表位在糖脂和糖蛋白之间的分布。PDMP抑制了糖脂抗原的表达，但不抑制通过免疫荧光和薄层色谱法检测的糖蛋白抗原的表达。就阶段特异性胚胎抗原1（SSEA1）而言，我们观察到其在糖脂和糖蛋白上均有表达。对PDMP抑制的抗性表明，在分化的人胚胎癌细胞和未分化的小鼠胚胎癌细胞的细胞表面上，糖蛋白携带了SSEA1的免疫显性形式。
