Olie R A, Fenderson B, Daley K, Oosterhuis J W, Murphy J, Looijenga L H
Centre d'Immunologie, Insern-CNRS de Marseille-Luminy, France.
Br J Cancer. 1996 Jul;74(1):133-40. doi: 10.1038/bjc.1996.328.
The glycolipid content of human non-seminomatous germ cell tumour cell lines correlates with their differentiation lineage. To analyse whether this reflects the situation in primary tumours, we studied five embryonal carcinomas, five yolk sac tumours and nine (mixed) non-seminomas, using thin-layer chromatography and carbohydrate immunostaining. We also analysed the glycolipid content of 19 seminomas to reveal their relationship with non-seminomas. Lactosylceramide (CDH) was detected in all embryonal carcinomas, but in fewer than half of the seminomas. Seminomas and embryonal carcinomas contained globoseries glycolipids, including globotriosylceramide (Gb3), globoside (Gb4), galactosy globoside (Gb5) and sialy1 galactosyl globoside (GL7). The lacto-series glycolipid Le(x) was found in all embryonal carcinomas, but only in one seminoma. Gangliosides GD3 and GT3 were detected in many seminomas, but rarely in embryonal carcinomas. Yolk sac tumours displayed a heterogeneous glycolipid profile. Compared with seminomas and pure embryonal carcinomas, differentiated non-seminomas had reduced levels of globo-series glycolipids, especially Gb3 and Gb5, whereas CDH, Le(x), GD3 and GT3 were found in the majority of cases. Thus, the glycolipid content of non-seminoma cell lines reflects the situation in primary tumours. Globo-series glycolipids are similarly expressed in seminomas and embryonal carcinomas. The expression of Gb3 and Gb5 is reduced in non-seminomas upon differentiation. Le(x) expression in non-seminomas, including embryonal carcinomas, allows discrimination from seminomas. Expression of gangliosides in seminomas might indicate their maturation from ganglioside-negative precursor cells. Reprogramming of these precursors would result in the formation of Le(x)-expressing embryonal carcinomas.
人类非精原性生殖细胞肿瘤细胞系的糖脂含量与其分化谱系相关。为分析这是否反映了原发性肿瘤的情况，我们使用薄层色谱法和碳水化合物免疫染色，研究了5例胚胎癌、5例卵黄囊瘤和9例（混合性）非精原细胞瘤。我们还分析了19例精原细胞瘤的糖脂含量，以揭示它们与非精原细胞瘤的关系。在所有胚胎癌中均检测到乳糖基神经酰胺（CDH），但在不到一半的精原细胞瘤中检测到。精原细胞瘤和胚胎癌含有球系列糖脂，包括球三糖基神经酰胺（Gb3）、球苷（Gb4）、半乳糖基球苷（Gb5）和唾液酸半乳糖基球苷（GL7）。乳糖系列糖脂Le(x)在所有胚胎癌中均有发现，但仅在1例精原细胞瘤中发现。神经节苷脂GD3和GT3在许多精原细胞瘤中被检测到，但在胚胎癌中很少见。卵黄囊瘤表现出异质性的糖脂谱。与精原细胞瘤和纯胚胎癌相比，分化型非精原细胞瘤中球系列糖脂水平降低，尤其是Gb3和Gb5，而在大多数病例中发现了CDH、Le(x)、GD3和GT3。因此，非精原细胞瘤细胞系的糖脂含量反映了原发性肿瘤的情况。球系列糖脂在精原细胞瘤和胚胎癌中表达相似。在非精原细胞瘤分化时，Gb3和Gb5的表达降低。非精原细胞瘤（包括胚胎癌）中Le(x)的表达有助于与精原细胞瘤区分开来。精原细胞瘤中神经节苷脂的表达可能表明它们从神经节苷脂阴性的前体细胞成熟而来。这些前体细胞的重编程将导致表达Le(x)的胚胎癌的形成。
