Dekker A J, Thal L J
Department of Neurosciences, University of California and Neurology Service, VA Medical Center, San Diego 92161.
Neurochem Res. 1993 Mar;18(3):277-83. doi: 10.1007/BF00969083.
Rats received a unilateral lesion of the nucleus basalis magnocellularis (NBM) by infusion of ibotenic acid. In addition, the dorsal raphe nucleus was lesioned by infusion of 5,7-dihydroxytryptamine (5,7-DHT). The release of acetylcholine (ACh), choline, serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) was measured in the frontal neocortex by means of microdialysis. Lesions of the NBM, but not the raphe nucleus, reduced the release of ACh significantly (-47%). The release of 5-HT and 5-HIAA was reduced by raphe lesions (-44% and -79%+), but not by NBM lesions. In no case did the combined lesion affect neurotransmitter release more than a single lesion. These results suggest that serotonergic projections from the dorsal raphe nucleus are not involved in tonic inhibition of ACh release in the neocortex.
通过注入鹅膏蕈氨酸，大鼠接受了基底大细胞核（NBM）的单侧损伤。此外，通过注入5,7-二羟基色胺（5,7-DHT）损伤了中缝背核。采用微透析法测量额叶新皮质中乙酰胆碱（ACh）、胆碱、5-羟色胺（5-HT）和5-羟吲哚乙酸（5-HIAA）的释放。NBM损伤而非中缝核损伤显著降低了ACh的释放（-47%）。中缝核损伤降低了5-HT和5-HIAA的释放（分别为-44%和-79%），但NBM损伤未产生此效果。在任何情况下，联合损伤对神经递质释放的影响都不超过单一损伤。这些结果表明，中缝背核的5-羟色胺能投射不参与新皮质中ACh释放的紧张性抑制。
